7Drug Combination Therapy is an international peer-reviewed, open access journal sponsored by TMR publishing group. DCT aims to publish unbiased original articles, reviews, letters, news and comment in all areas of drug combination therapy. DCT is dedicated to report the latest advancements and the research done in drug combination along with all the relevant fields, including but not limited to prescription compatibility studies, disassembled formulae studies, drug compatibility synergetic/antergic mechanisms, drug incompatibility/adverse reactions, combination therapy for specific populations, integrated pharmacology studies, system/network pharmacology, big data analysis, systematic evaluation/meta-analysis, omics research, natural products chemistry, pharmaceutical analysis, etc. In order to focus on breakthrough research in a field, DCT insist on publishing special issues around a topic related to drug combination therapy. DCT has been included in EuroPub, Google Scholar, CNKI Scholar, VIP Data, Superstar Journals Database and Traditional Medicine Research Data Expanded.
Backgroud: To explore the effects of Jianpi Bushen Recipe on the epithelial-mesenchymal transition (EMT) of nude mice with transplanted human liver cancer SK-HEP-1 cells. Methods: To establish models with transplanted liver cancer cells, nude mice were divided randomly into three groups: blank group, traditional Chinese medicine (TCM) group and control group. They were given normal saline, Jianpi Bushen Recipe and solafeni through administration by gavage for four weeks. The expression of TGF-β and twist mRNA were detected by real-time PCR. The expression of E-cadherin was detected by Western blot. Results: Compared with the blank group, the expression of transforming growth factor-β (TGF-β) mRNA and twist mRNA in the TCM group and the control group decreased significantly (P < 0.05). There was no significant difference between the two groups (P > 0.05). The expression of E-cadherin protein in TCM group and control group increased significantly (P < 0.01). There was no significant difference between the two groups (P > 0.05). Conclusion: Jianpi Bushen Recipe can inhibit the EMT of liver cancer SK-HEP-1 cells in vivo by down-regulating the expression of TGF-β/twist signaling and indirectly up-regulating E-cadherin expression in transplanted liver cancer tissue, indicating the potential of Jianpi Bushen Recipe to inhibit invasion and metastasis of liver cancer.
Hong-Jie Liu, You-Ying Lai, Zhi-Fen Han, Jian-Jie Chen, Xin-Wen Ma
Backgroud: To evaluate the clinical efficacy and safety of Dachaihu Decoction in the treatment of acute pancreatitis (AP) with the traditional Chinese medicine (TCM) syndrome of liver qi depression and liver-gallbladder dampness-heat. Methods: We searched randomized controlled trials of Dachaihu Decoction in the treatment of AP with the TCM syndrome of liver qi depression and liver-gallbladder dampness-heat from databases in CNKI, WanFang, VIP, Embase, PubMed, and Cochrane Library (from established to August 2019). All the retrieved documents were imported into the Noteexpress software for screening and management, and the included documents were evaluated for quality and data extracted. Statistical analysis was performed with RevMan (version 5.3). Results: A total of 132 articles were retrieved and 8 studies were included to analysis, including 679 patients. Quality evaluation of included studies suggested that the quality of the literatures is generally not high. Meta-analysis showed that the total clinical effective rate of AP was higher in the treatment group than in the control group [OR = 5.00, 95% CI (2.86, 8.73)], and the difference was statistically significant (P < 0.00001). The patient’s abdominal pain relief time [MD = -0.57, 95% CI (-0.86, -0.27), P = 0.0002], bloating relief time [MD = -0.71, 95% CI (-1.05, -0.37), P = 0.04], time of serum amylase returning to normal [MD = -1.00, 95% CI (-1.60, -0.40), P = 0.001] and time of urinary amylase returning to normal [MD = -1.62, 95% CI (-2.88, -0.37), P = 0.01] all lower than the control group. But there was no statistically significant difference between the treatment group and the control group at the first defecation time [MD= -0.86, 95% CI (-1.75, -0.03), P = 0.06]. The patient’s serum TNF-α levels on the 3rd [MD = -28.53, 95% CI (-49.57, -7.49), P = 0.008] and 7th day [MD = -26.13, 95% CI (-49.76, -2.50), P = 0.03] in the treatment group were statistically lower in the treatment group than in the control group. Similarly, the patient’s serum IL-6 levels on the 3rd [MD= -6.62, 95% CI (-12.49, -0.75), P = 0.03] and 7th day [MD = -11.98, 95% CI (-22.25, -1.71), P = 0.02] were also statistically lower in the treatment group than in the control group. No serious complications or adverse reactions were observed. Conclusion: Dachaihu Decoction combined with western medicine in the treatment of AP with the TCM syndrome of liver qi depression and liver-gallbladder dampness-heat can improve clinical efficacy compared with western medicine alone. In addition, the combination therapy method is safe and can be used for syndrome differentiation of AP patients in the clinic.
Nephrotic syndrome (NS) is a common refractory clinical disease that can develop into end-stage renal disease (ESRD) if cannot be controlled. At present, in Western medicine, glucocorticoid or immunosuppressant drugs with heavy dose and long course are used for the therapy of NS. There are still few reports on the combined treatment of traditional Chinese medicine and low-dose glucocorticoid for NS. Here, we reported a case of a 48-year-old man diagnosed with NS, accompanied by severe pulmonary infection, abundant proteinuria, and pleural effusion. Integrated traditional Chinese and Western medicine was used as the therapeutic regimen of this patient. Traditional Chinese medicine (TCM) mainly focused on invigorating spleen and kidney, promoting blood circulation, removing blood stasis and promoting diuresis, and Chinese patent medicines or TCM immunosuppressants were given to consolidate the treatment. In Western medicine, small dose and short course glucocorticoids combined with symptomatic treatment were adopted. Delightfully, proteinuria subsided after 5 months treatment with integrated TCM and Western medicine and all laboratory reports were negative after 10 months, which has been normal so far (July 2019). This case report indicates that the combination of TCM and low-dose glucocorticoid is effective in curing NS, with low recurrence rate and few side effects.