7Drug Combination Therapy (DCT) is an international peer-reviewed, open access journal sponsored by TMR publishing group. DCT aims to publish unbiased original articles, reviews, letters, news and comment in all areas of drug combination therapy. DCT is dedicated to report the latest advancements and the research done in drug combination along with all the relevant fields, including but not limited to prescription compatibility studies, disassembled formulae studies, drug compatibility synergetic/antergic mechanisms, drug incompatibility/adverse reactions, combination therapy for specific populations, integrated pharmacology studies, system/network pharmacology, big data analysis, systematic evaluation/meta-analysis, omics research, natural products chemistry, pharmaceutical analysis, etc. In order to focus on breakthrough research in a field, DCT insist on publishing special issues around a topic related to drug combination therapy. DCT has been included in CAS databases, EuroPub, J-Gate, Google Scholar, CNKI Scholar, Baidu Scholar, VIP Data, Superstar Journals Database and Traditional Medicine Research Data Expanded.
Background: To analyze the medication and methods of ancient traditional Chinese medicine for the prevention of plague and seasonal pathogen in order to reduce the spread of infectious diseases such as flu and protect the susceptible people. Methods: Search the medication and the methods for preventing plague and seasonal pathogen in the Chinese Medical Code, classify and summarize the methods, perform frequency analysis and efficacy cluster analysis on Chinese medicines of involved medicines. Results: The methods of preventing ancient plague were as follows: burning and fumigating Chinese medicines, medicating nostrils, wearing bags filled with Chinese medicines, oral Chinese medicines and applying medicines to skin. The three main ways for drugs to work were external medication, nostril inhalation, and internal absorption. The Chinese medicines for preventing plague are mostly warm in nature and pungent in flavour, belonging to the spleen, stomach, liver, lung meridian. The three categories of drugs obtained by cluster analysis are C1: drugs for facilitating lung, relieving exterior and dissipating cold, such as Cangzhu (Atractylodis rhizoma)-Qianghuo (Notopterygh rhizoma et radix)-Xixin (Asari radix et rhizoma)-Baizhi (Angelicae dahuricae radix)-Fangfeng (Saposhnikoviae radix)-Zaojiao (Gleditsiae sinensis fructus); C2: drugs for promoting blood circulation and removing blood stasis, such as Xionghuang (Realgar)-Zhusha (Cinnabaris)-Dahuang (Rhei radix rhizoma)-Chuanxiong (Chuanxiong rhizoma)-Jiegeng (Platycodonis radix); C3: drugs for replenishing Qi (it is the most basic element that constitutes human body and maintains life activities in traditional Chinese medicine) and tonifying the spleen, such as Gancao (Glycyrrhizae radix et rhizoma)-Dazao (Jujubae fructus)-Ganjiang (Zingiberis rhizoma)-Rougui (Cinnamomi cortex)-Gansong (Nardostachyos radix et rhizoma)-Chenpi (Citri reticulatae pericarpium)-Baizhu (Atractylodis macrocephalae rhizoma). Conclusion: Through analysis the medication and methods of ancient traditional Chinese medicine for the prevention of plague, we can provide reference for modern research from the original plague prevention methods, and make an important contribution to the use of traditional Chinese medicine in the prevention and treatment of epidemic infectious diseases in modern clinic.
Wei-Sen Fan, Wen-Hui Zhang, Yun-Sheng Liu, Xiao-Mei Feng, Zhen-Gao Sun
Background: To verify the antipyretic effect and mechanism of Chai Ge fever relief oral liquid, and to provide evidence for the clinical application of Chai Ge fever relief oral liquid. Methods: By designing three groups of experimental models of yeast-induced rat fever model, endotoxin-induced rabbit fever model, and para-influenza virus-induced rabbit fever model, the antipyretic effect and mechanism of Chai Ge fever relief oral liquid were studied by measuring body temperature before and after treatment and the expressions of protein kinase A (PKA), protein kinase C, arginine vasopressin (AVP), prostaglandin E2 and cyclic adenosine monophosphate (cAMP) in hypothalamus after administration. Results: In the yeast-induced fever model of rats, the body temperature and PKA expression levels in different dose groups of Chai Ge fever relief oral liquid were significantly different from those of the model group 1–3h after treatment. In the rabbit fever model induced by endotoxin, different dosage groups of Chai Ge fever relief oral liquid have obvious antipyretic effect on 1–3h after the model is established, and can significantly reduce the expression of AVP and cAMP in hypothalamus. In the para-influenza virus-induced rabbit fever model, each dose group of Chai Ge fever relief oral liquid has obvious antipyretic effect on 1–2h after the model is established. Conclusion: Chai Ge fever relief oral liquid has good antipyretic effect on fever models induced by yeast, endotoxin and parainfluenza by regulating the expression of PKA, AVP, and cAMP.
Shan-Shan Guo, Ya-Xin Wang, Ying-Jie Gao, Sai-Wei Lu, Xiao-Lan Cui
Background: To systematically evaluate the clinical efficacy of tonifying kidney and soothing liver method in the treatment of polycystic ovary syndrome (PCOS). Methods: The literatures of randomized controlled trials (RCTs) on the treatment of PCOS with traditional Chinese medicine (TCM) published at home and abroad until March 2020 were retrieved, and RCTs on the treatment of PCOS with tonifying kidney and soothing liver method were collected. According to the systematic evaluation method of the Cochrane Collaboration Network, RevMan5.3 software was used to perform quality evaluation and data extraction for meta-analysis of tests that met the inclusion criteria. Results: A total of 30 eligible RCTs were included, including 2244 patients. Meta-analysis results show that the method of tonifying the kidney and soothing liver has certain advantages in the treatment of PCOS. Compared with Western medicine alone group, tonifying the kidney and soothing liver TCM method alone or combined with Western medicine could improve the total effective rate [OR = 3.52, 95% CI (2.67, 4.63), I2 = 0%, P < 0.00001]. It is superior to the control group in improving ovulation rate [OR = 2.01, 95% CI (1.46, 2.78), I2 = 0%, P < 0.0001], pregnancy rate [OR = 2.82, 95% CI (2.28, 3.49), I2 = 0%, P < 0.00001], reducing LH [MD = -2.65, 95% CI (-3.43, -1.88), I2 =92%, P < 0.00001] and ovarian stimulating hormone levels [MD = -0.52, 95% CI (-0.85, -0.18), I2 = 94%, P = 0.002]. There was no statistical difference in reducing serum testosterone levels [SMD = -0.56, 95% CI (-1.22, 0.09), I2 = 96%, P = 0.09] and improving TCM symptoms [MD = -0.09, 95% CI (-4.75, 4.56), I2 = 97%, P = 0.97]. Conclusion: The method of tonifying the kidney and soothing liver has a certain effect in treating polycystic ovary syndrome, but the evidence is still very limited, and a large sample of high-quality randomized controlled studies is still needed in order to make a more rigorous evaluation.
Background: To investigate the mechanism of Xiaozheng decoction in treatment of bladder cancer based on network pharmacology. Methods: Based on the Tradictional Chinese Medicine Systems Pharmacology Database(TCMSP), the active compositions of Xiaozheng decoction were screened. The targets of active components were obtained from TCMSP, Swiss Target Prediction, and STITCH database. By mapping the disease targets of bladder cancer obtained from the DisGeNET and Genecards databases, the potential targets of Xiaozheng decoction for bladder cancer were obtained. The active components of Xiaozheng decoction-targets network was constructed using the Cytoscape software. The target protein interaction (PPI) network was constructed by using the String online platform, which was visualized by Cytoscape software and analyzed by network topology to obtain the key targets of Xiaozheng decoction. GO bioprocess enrichment analysis and KEGG pathway enrichment analysis were performed on key targets of the Xiaozheng decoction by DAVID database. Results: A total of 68 active components and 255 potential targets of Xiaozheng decoction in the treatment of bladder cancer were retrieved and screened out. The key targets were enriched and analyzed by the GO biological pathway, and 135 pathways were obtained, which involved transcriptional process, platelet activation, cell proliferation, and apoptosis. KEGG pathway enrichment analysis revealed 95 pathways, mainly involving cancer pathway, bladder cancer pathway, MAPK signaling pathway, PI3K-Akt signaling pathway, and VEGF signaling pathway. Conclusion: The result of this study showed that Xiaozheng decoction may play its therapeutic role through multiple components, multiple targets and multiple pathways, and preliminarily explored the mechanism of Xiaozheng decoction in the treatment of bladder cancer, laying a foundation for subsequent experimental studies.