Please wait a minute...
2TMR Integrative Medicine  2019, 3: e19012.       DOI: 10.12032/TMRIM201903012
In-vitro pharmacological evaluation of Sulforaphane from Brassica oleracea
Sodum Nalini1, Shaik Chand Basha2,*(), TS Mohamed Saleem3
1Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, India
2Department of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, India
3Department of Pharmacology, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, India.
Download: HTML     PDF(424KB)
Export: BibTeX | EndNote (RIS)      


Sulforaphane shows significant anti-arthritic activity through in-vitro screening models and the activity was observed as concentration dependent against bovine serum albumin (BSA), egg albumin denaturation assay.


Objective: Sulforaphane has numerous Pharmacological and therapeutic effects like anti-oxidant, anti-cancer, anti-diabetic, anti-arthritic, anti-ulcer, anti-viral. The present study was designed to evaluate the anti-arthritic activity of Sulforaphane by in-vitro screening methods.Methods The anti-arthritic activity of Sulforaphane was investigated by protein denaturation assay by using Bovine serum albumin (BSA) and egg albumin. Sulforaphane used in various concentration (10, 50, 100, 250, 500 μg/ml) against both the methods and Diclofenac sodium was used as reference standard.Results The anti-arthiritic activity sulforaphane was directly proportional to inhibition of denaturation of albumin. Sulforaphane shows concentration dependent inhibition activity in both bovine serum albumin and egg albumin assay. The maximum inhibition was observed in the concentration of 500 μg/ml with 92.8% inhibition for BSA and 96.3% for egg albumin respectively.Conclusion Sulforaphane have significant anti-arthritic activity accessed by BSA denaturation and egg albumin denaturation assay. Based on present finding future direction also planned to conform the activity by using well established in-vivo methods.

Key wordsSulforaphane      Anti-arthritic activity      Protein denaturation methods     
Published: 22 June 2019
Corresponding Authors: Chand Basha Shaik   
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
Articles by authors
Sodum Nalini
Shaik Chand Basha
TS Mohamed Saleem
Related Articles
No related articles found!
Cite this article:

Sodum Nalini, Shaik Chand Basha, TS Mohamed Saleem. In-vitro pharmacological evaluation of Sulforaphane from Brassica oleracea. 2TMR Integrative Medicine, 2019, 3: e19012.  

URL:     OR 3/88888

Figure 1 Chemical structure of Sulforaphane
Figure 2 Anti-arthritic activity of Sulforaphane by bovine serum albumin method
Figure 3 Anti-arthritic activity of Sulforaphane by egg albumin method
1.   Choudhary M, Kumar V, Malhotra H,et al. Medicinal plants with potential anti-arthritic activity. J Intercultural Ethanopharmacology 2015, 4: 12-18.
2.   Khanna D, Sethi G, Ahn KS, et al. Natural products as a gold mine for arthritis treatment. Current Opinion Pharmacology 2007, 7: 344-351.
3.   Chunxia C, Peng Z, Huifang P, et al. Extracts of Arisaema rhizomatum C.E.C. Fischer attenuate inflammatory response on collagen-induced arthritis in BALB/c mice. J Ethnopharmacology 2011, 133: 573-582.
4.   Tripathy S Pradhan, Njana D.Anti-inflammatory and anti-arthritic potential of Ammania baccifera Linn. Int J Pharmaceutical Bio Sci 2010, 1: 1-7.
5.   Firestein GS.Evolving concepts of rheumatoid arthritis. Nature 2003, 423: 356-361.
6.   Lin J, Zhang W, Jones A, et al. Efficacy of topical non-steroidal anti-inflammatory drugs in the treatment of osteoarthritis: meta-analysis of randomized controlled trials. BMJ 2004, 1: 1-6.
7.   Chand Basha S, Rajesh Babu K, Madhu M, et al. In-vitro anti diabetic activity of Sulphoraphane. PTB Reports 2017, 3: 47-49.
8.   Rahman H, Eswaraiah C, Dutta M.In-vitro anti-inflammatory and anti-arthritic activity of oryza sativa var. Joha American-Eurasian J Agriculture Environmental Sci 2015, 15: 115-121.
9.   Dutta SK, Basu SK, Sen KK.Binding of diclofenac sodium with bovine serum albumin at different temperatures, pH and ionic strengths. Indian J Exp Biol 2006, 44: 123-127.
10.   Murugananthan G, Sudheer KG, Sathya CP, et al. Anti-arthritic and anti-inflammatory constituents from medicinal plants. J Appl Pharm Sci 2013, 3: 161-164.
11.   Reddy VJS, Rao PGD, Lakshmi GR.A review on antiarthritic activity of some medicinal plaants. J Glob Trends Pharm Sci 2014, 5: 2061-2073.
12.   Zubair M, Anwar F, Shahid SA.Effect of extraction solvents on phenolics and antioxidant activity of selected varieties of pakistani rice. J Agric Biol 2012, 14: 935-940.
13.   Atawodi SE, Yakubu OE, Umar IA.Antioxidant and hepatoprotective effects of parinari curatellifolia root. Int J Agriculrure Biol 2013, 15: 523-528.
14.   Saeed N, Khan MR, Shabbir M.Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts torilis leptophylla L. BMC Complement Altern Med 2012, 12: 221.
15.   Dzoyem JP, Kuete V, McGaw LJ, et al. The 15-lipoxygenase inhibitory, antioxidant, antimycobacterial activity and cytotoxicity of fourteen ethnomedicinally used African spices and culinary herbs. [J] Ethnopharmacol. 2014, 156: 1-8.
16.   Gray SG.The potential of epigenetic compounds in treating diabetes. Epigenetics in Human Disease, Academic Press, 2012.
17.   Manvarl MN, Desai TR.In-vitro anti-inflammatory and anti-arthritic activities of fruits of vernonia anthelmintica willd. Asian J Pharmacy 2014, 4: 186-188.
No related articles found!