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2TMR Integrative Medicine  2019, 3: e19012.       DOI: 10.12032/TMRIM201903012
    
In-vitro pharmacological evaluation of Sulforaphane from Brassica oleracea
Sodum Nalini1, Shaik Chand Basha2,*(), TS Mohamed Saleem3
1Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, India
2Department of Pharmaceutical Chemistry, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, India
3Department of Pharmacology, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, India.
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Highlights:

Sulforaphane shows significant anti-arthritic activity through in-vitro screening models and the activity was observed as concentration dependent against bovine serum albumin (BSA), egg albumin denaturation assay.

Abstract

Objective: Sulforaphane has numerous Pharmacological and therapeutic effects like anti-oxidant, anti-cancer, anti-diabetic, anti-arthritic, anti-ulcer, anti-viral. The present study was designed to evaluate the anti-arthritic activity of Sulforaphane by in-vitro screening methods.Methods The anti-arthritic activity of Sulforaphane was investigated by protein denaturation assay by using Bovine serum albumin (BSA) and egg albumin. Sulforaphane used in various concentration (10, 50, 100, 250, 500 μg/ml) against both the methods and Diclofenac sodium was used as reference standard.Results The anti-arthiritic activity sulforaphane was directly proportional to inhibition of denaturation of albumin. Sulforaphane shows concentration dependent inhibition activity in both bovine serum albumin and egg albumin assay. The maximum inhibition was observed in the concentration of 500 μg/ml with 92.8% inhibition for BSA and 96.3% for egg albumin respectively.Conclusion Sulforaphane have significant anti-arthritic activity accessed by BSA denaturation and egg albumin denaturation assay. Based on present finding future direction also planned to conform the activity by using well established in-vivo methods.



Key wordsSulforaphane      Anti-arthritic activity      Protein denaturation methods     
Published: 22 June 2019
Corresponding Authors: Chand Basha Shaik   
E-mail: schandbasha20@gmail.com
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Sodum Nalini
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Cite this article:

Sodum Nalini, Shaik Chand Basha, TS Mohamed Saleem. In-vitro pharmacological evaluation of Sulforaphane from Brassica oleracea. 2TMR Integrative Medicine, 2019, 3: e19012.  

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https://www.tmrjournals.com/im/EN/10.12032/TMRIM201903012     OR     https://www.tmrjournals.com/im/EN/Y2019/V3/IVolume 3/88888

Figure 1 Chemical structure of Sulforaphane
Figure 2 Anti-arthritic activity of Sulforaphane by bovine serum albumin method
Figure 3 Anti-arthritic activity of Sulforaphane by egg albumin method
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