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2TMR Integrative Medicine  2019, 3: e19015.       DOI: 10.12032/TMRIM201903015
    
Study on immunological characteristics of T lymphocyte in peripheral blood from patients with Sjogren's syndrome
Rong Bi1, Sheng-Ping Zeng2, Bo Wen3, Chun-Bo Wang4, Ji-Hong Tan5
1Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China. 2Affiliated Hospital of Chengdu University of Traditional Chinese Medicine/Chengdu Gaoxin Huisheng Rheumatism Hospital, Chengdu, China. 3Beijing Traditional Chinese Medicine Hospital Affiliated to Capital Medical University, Chengdu, China. 4Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Chengdu, China. 5Chengdu Yingdu District Hospital of Traditional Chinese Medicine, Chengdu, China.
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Objective: To study on in immunological characteristics of T lymphocyte in peripheral blood from patients with Sj?gren's syndrome (SS), provide the theory basis for the further research of Traditional Chinese medicine (TCM) targeted therapy. Methods: T lymphocyte subsets in peripheral blood from SS, RA and normal control groups were tested with various monoclonal antibodies and multicolor flow cytometry. T lymphocyte subsets were tested in peripheral blood from SS patients of different TCM Syndromes. The relationship between T lymphocyte subsets and disease activity was measured by erythrocyte sedimentation rate (SR)and serum IgG. Results: The percentage of CD4+T cells and the ration of CD4+ / CD8+ T cells in peripheral blood from SS patients decreased significantly, and the percentage of CD8+T cells increased than that in control groups (P < 0.05). Compared with patients in inactive period, the percentage of CD4+ T cells in peripheral blood from SS patients in the active stage increased (P > 0.05), the percentage of CD8+T cells decreased (P > 0.05), and the ration of CD4+ / CD8+ T cells increased (P < 0.05). Compared with control groups, the percentage of CD69 expressed on T cells in peripheral blood from SS patients decreased, and the percentage of CD95's expression increased (P < 0.05). The percentage of CD69CD95's expression also increased (P > 0.05). There were no differences among CD4+ T cells, CD8+ T cells and CD4+ / CD8+ in peripheral blood from SS patients of different TCM Syndromes (P > 0.05). CD69 and CD95's expression on SS patients of different TCM Syndromes was no significantly different (P > 0.05). T cell receptor Vβ (TCR Vβ) subfamilies gene in peripheral blood from SS patients and control groups were expressed in all. Compared with control groups, Vβ 5.3, Vβ 13.1, Vβ 12, Vβ 7.2 had significant differences (P < 0.05). Comparison of different TCM Syndromes in SS group: Vβ 13.1 and Vβ 14 were significantly different (P < 0.05). Conclusion: There was dysimmunity on T lymphocyte subsets in peripheral blood of SS patients, which was related to disease activity; apoptosis after activation of autoreactive T cell may be induced by Chinese medicine; TCR Vβ subfamilies of different TCM syndromes were different, which noted that different autoreactive T cells of abnormal activation tended to result in different expression of TCM Different syndromes.


Key wordsSjogren's syndrome      T lymphocyte subsets      Flow cytometry      Monoclonal antibody      T cell receptor Vβ gene     
Published: 09 August 2019
Corresponding Authors: Sheng-Ping Zeng, Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu Gaoxin Huisheng Rheumatism Hospital, Chengdu, China.    
E-mail: 1761210020@qq.com
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Cite this article:

Rong Bi, Sheng-Ping Zeng, Bo Wen, Chun-Bo Wang, Ji-Hong Tan. Study on immunological characteristics of T lymphocyte in peripheral blood from patients with Sjogren's syndrome. 2TMR Integrative Medicine, 2019, 3: e19015.  

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https://www.tmrjournals.com/im/EN/10.12032/TMRIM201903015     OR     https://www.tmrjournals.com/im/EN/Y2019/V3/IVolume 3/88888

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