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Editors-in-Chief: L Xiong, PhD
ISSN 2521-3415
2TMR Integrative Medicine is a peer reviewed, open access, and serialization open access (SOA) journal. It is dedicated to report the research progress and discusses the idea and method in clinical efficacy, action mechanism and theoretical research on integrative medicine, including evidence research, integrative research, alternative research, holistic research modernization research and other researches on integrative medicine around the world. It set up basic research, clinical research, case reports, review and theoretical discussion. There are hot topics include pharmacology research, toxicology research, theoretical research, clinical research, meta analysis, etc. It seeks to promote international communication focusing on the latest developments, trends, experiences and achievements on integrative medicine. It has been included in DOAJ, ScienceOpen, China Science and Technology Journal Database (CSTJ), and Google Scholar. ... More

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Can Chinese herbal medicine improve the pregnancy outcomes of patients with poor ovarian response? —a systematic review and meta-analysis of randomized controlled trials
Du Li, Shu-Yong Zhang, Dian-Rong Song
2TMR Integrative Medicine. 2019, 3: e19019.   https://doi.org/10.12032/TMRIM201903019
Abstract ( 10 )     PDF (1246KB) ( 5 )  
Poor response to ovarian stimulation which results in low pregnancy rate has been a major challenging in assisted reproductive technology. Chinese herbal medicine (CHM) has been used as a supplementary or an alternative intervention in improving pregnancy rate in women with poor ovarian response (POR). This systematic review was aimed to assess the clinical effectiveness of Chinese herbal medicine for the treatment of POR. We searched Embase, PubMed, China national knowledge infrastructure (CNKI), VIP Database, Chinese biomedical database (CBM), Wan Fang database and the Cochrane library from January 2011 to March 2018. Study quality assessment and meta­analyses were performed according to the Cochrane recommendations. 16 trials including 1245 ovulation-promoting cycles were evaluated. The analysis showed that CHM can increase the pregnancy rate (RR = 1.80, 95% CI (1.41-2.29), P < 0.001), while decrease cycle cancellation (RR= 0.66, 95% CI (0.45-0.97), P = 0.04). Conclusion: CHM presented a promising effectiveness in women with POR as a supplementary or an alternative intervention in IVF/ICSI.
Modulation of various enzyme activities and lipid peroxidation by Schima wallichii (DC.) Korth in mice bearing Dalton's ascitic lymphoma
Ganesh Chandra Jagetia, K. Lalhminghlui
2TMR Integrative Medicine. 2019, 3: e19018.   https://doi.org/10.12032/TMRIM201903018
Abstract ( 25 )     PDF (663KB) ( 16 )  
The effect of ethanol extract of Schima wallichii (SWE) was studied in the Swiss albino mice bearing Dalton's ascitic lymphoma (DAL) after nine consecutive days administration. The tumor cells were then extracted at 2, 4, 8, 12 and 24 h post drug treatment for the estimation of glutathione (GSH), glutathione-s-transferase (GST), catalase (CAT), superoxide dismutase (SOD), and lipid peroxidation. We found that tumor cells had higher amount of GSH, GST, CAT and SOD; Treating tumorized mice with SWE reduced the level of GSH and the activities of GST, CAT and SOD in a time dependent manner up to 24h post-treatment followed by an increased lipid peroxidation. The liver function test revealed that aspartate aminotransferase (AST), and alanine aminotransferase (ALT) did not increase, indicating that it did not exert toxic effects on the liver. The assay of uric acid and creatinine were also in normal range. Our study indicated that tumor cells had higher amount of GSH, GST, CAT and SOD; the treatment of SWE reduced the level of GSH, GST, CAT and SOD, increased the lipid peroxidation at the same time, which may be responsible for its tumor cell killing effects. Meanwhile, it did not produce biochemical stress in the liver of tumorized mice.
Traditional Chinese non-drug therapies for cancer pain treatment
Xun Liu, Zhu Yang, Feng-Xi Long, Jin-Lin Wu, Dong-Xin Tang
2TMR Integrative Medicine. 2019, 3: e19017.   https://doi.org/10.12032/TMRIM201903017
Abstract ( 41 )     PDF (293KB) ( 24 )  

Cancer pain is the most common and serious symptom of cancer, which seriously affects the quality of life of cancer patients. the most widely used three-step analgesia has received good effects, but the toxic side effects at the same time can be ignored. As a complementary therapy increasingly accepted around the world, traditional Chinese medicine (TCM) also plays an important role in cancer pain treatment. Traditional Chinese non-drug therapy has a good effect for cancer pain. In this mini-review, the commonly used traditional Chinese non-drug therapies such as acupuncture, moxibustion, acupoint catgut embedding and injection, massage, ear point pressing with coxherb seeds therapy, traditional music therapy were introduced.

Study on immunological characteristics of T lymphocyte in peripheral blood from patients with Sjogren's syndrome
Rong Bi, Sheng-Ping Zeng, Bo Wen, Chun-Bo Wang, Ji-Hong Tan
2TMR Integrative Medicine. 2019, 3: e19015.   https://doi.org/10.12032/TMRIM201903015
Abstract ( 45 )     PDF (720KB) ( 5 )  
Objective: To study on in immunological characteristics of T lymphocyte in peripheral blood from patients with Sj?gren's syndrome (SS), provide the theory basis for the further research of Traditional Chinese medicine (TCM) targeted therapy. Methods: T lymphocyte subsets in peripheral blood from SS, RA and normal control groups were tested with various monoclonal antibodies and multicolor flow cytometry. T lymphocyte subsets were tested in peripheral blood from SS patients of different TCM Syndromes. The relationship between T lymphocyte subsets and disease activity was measured by erythrocyte sedimentation rate (SR)and serum IgG. Results: The percentage of CD4+T cells and the ration of CD4+ / CD8+ T cells in peripheral blood from SS patients decreased significantly, and the percentage of CD8+T cells increased than that in control groups (P < 0.05). Compared with patients in inactive period, the percentage of CD4+ T cells in peripheral blood from SS patients in the active stage increased (P > 0.05), the percentage of CD8+T cells decreased (P > 0.05), and the ration of CD4+ / CD8+ T cells increased (P < 0.05). Compared with control groups, the percentage of CD69 expressed on T cells in peripheral blood from SS patients decreased, and the percentage of CD95's expression increased (P < 0.05). The percentage of CD69CD95's expression also increased (P > 0.05). There were no differences among CD4+ T cells, CD8+ T cells and CD4+ / CD8+ in peripheral blood from SS patients of different TCM Syndromes (P > 0.05). CD69 and CD95's expression on SS patients of different TCM Syndromes was no significantly different (P > 0.05). T cell receptor Vβ (TCR Vβ) subfamilies gene in peripheral blood from SS patients and control groups were expressed in all. Compared with control groups, Vβ 5.3, Vβ 13.1, Vβ 12, Vβ 7.2 had significant differences (P < 0.05). Comparison of different TCM Syndromes in SS group: Vβ 13.1 and Vβ 14 were significantly different (P < 0.05). Conclusion: There was dysimmunity on T lymphocyte subsets in peripheral blood of SS patients, which was related to disease activity; apoptosis after activation of autoreactive T cell may be induced by Chinese medicine; TCR Vβ subfamilies of different TCM syndromes were different, which noted that different autoreactive T cells of abnormal activation tended to result in different expression of TCM Different syndromes.
Prevention and treatment of traditional Chinese medicine for elderly patients with colorectal cancer
Jia Wang, Zhu Yang, Feng-Xi Long, Jin-Lin Wu, Ting Yu, Xian-Man Wei, Xun Liu, Dong-Xin Tang
2TMR Integrative Medicine. 2019, 3: e19016.   https://doi.org/10.12032/TMRIM201903016
Abstract ( 35 )     PDF (436KB) ( 19 )  
As China's population ages, the incidence of colorectal cancer in the elderly has increased. At present, colorectal cancer depends on surgery, radiotherapy and chemotherapy. Due to the characteristics of the elderly, postoperative recovery is slow, prone to postoperative complications and difficult to tolerate radiotherapy and chemotherapy have become the main problem hindering the disease. Based on the theory of prevention, the use of drugs from the perspective of constitution and etiology can prevent the occurrence of colorectal cancer in the elderly. Traditional Chinese medicine (TCM) combined with radiotherapy and chemotherapy can alleviate adverse reactions and improve treatment efficacy. TCM with surgical treatment can reduce the occurrence of postoperative complications and promote postoperative recovery. For patients with advanced colorectal cancer, TCM can play a role in fighting cancer and improving the quality of life of patients. For patients in stable phase, TCM can prevent tumor recurrence and metastasis.
Syndrome differentiation of Zang-Fu for Tourette syndrome in children
Zhao Mu, Teng Huang, Zhan-Ling Zhang
2TMR Integrative Medicine. 2019, 3: e19013.   https://doi.org/10.12032/TMRIM201903013
Abstract ( 2 )     PDF (350KB) ( 4 )  
As a kind of psychoneurotic disease, the incidence of Tourette syndrome (TS) is increasing gradually in recent years. Modern medicine for the pathogenesis of this disease has not been clear, in the treatment is mainly symptomatic treatment. Based on syndrome differentiation and treatment, traditional medicine treats the unique system of etiology and pathogenesis of the disease, and achieves remarkable results in treatment. Chinese medicine treatment of children's diseases generally from the viscera dialectical. In this paper, we summarize the clinical experience of the treatment of pediatric TS by syndrome differentiation of viscera in order to provide reference for the clinical treatment and research of pediatric TS.
The effect of music therapy on rehabilitation of children with cerebral palsy: a meta-analysis
Yu-Hui Ma, Li-Na Zhang, Qian Cui, Xiao-Ming Jin, Guo-Wei Zhang
2TMR Integrative Medicine. 2019, 3: e19014.   https://doi.org/10.12032/TMRIM201903014
Abstract ( 40 )     PDF (619KB) ( 16 )  
Objective: To evaluate the clinical efficacy of music therapy in the treatment of children with cerebral palsy by systematic review and meta-analysis. Methods: A comprehensive search of China Knowledge Network, Wan fang, VIP database and foreign databases PubMed and Cochrane Library was conducted from the setup of database to March 2019. After reading and screening them carefully, 14 qualified literates were got. Then extracted relevant data, and evaluated the quality and risk bias with Revman5.3.3 software. Results: There is no significant difference between music therapy in improving clinostatism and the ability of rolling over (P > 0.05). However, music therapy has significant difference in improving sitting, running, and jumping movements, and it can also relieve muscle spasm, improve muscle tension and stabilize children’s emotions in the course of treatment and the P values of which were all less than 0.05. Conclusion: The music therapy has a certain effect for the treatment of pediatric cerebral palsy, especially in improving children’s gross and fine motor abilities, which has a certain clinical guiding significance for rehabilitation of children with cerebral palsy.
In-vitro pharmacological evaluation of Sulforaphane from Brassica oleracea
Sodum Nalini, Shaik Chand Basha, TS Mohamed Saleem
2TMR Integrative Medicine. 2019, 3: e19012.   https://doi.org/10.12032/TMRIM201903012
Abstract ( 64 )   HTML ( 4 )     PDF (424KB) ( 30 )  

Highlights:

Sulforaphane shows significant anti-arthritic activity through in-vitro screening models and the activity was observed as concentration dependent against bovine serum albumin (BSA), egg albumin denaturation assay.

Abstract

Objective: Sulforaphane has numerous Pharmacological and therapeutic effects like anti-oxidant, anti-cancer, anti-diabetic, anti-arthritic, anti-ulcer, anti-viral. The present study was designed to evaluate the anti-arthritic activity of Sulforaphane by in-vitro screening methods.Methods The anti-arthritic activity of Sulforaphane was investigated by protein denaturation assay by using Bovine serum albumin (BSA) and egg albumin. Sulforaphane used in various concentration (10, 50, 100, 250, 500 μg/ml) against both the methods and Diclofenac sodium was used as reference standard.Results The anti-arthiritic activity sulforaphane was directly proportional to inhibition of denaturation of albumin. Sulforaphane shows concentration dependent inhibition activity in both bovine serum albumin and egg albumin assay. The maximum inhibition was observed in the concentration of 500 μg/ml with 92.8% inhibition for BSA and 96.3% for egg albumin respectively.Conclusion Sulforaphane have significant anti-arthritic activity accessed by BSA denaturation and egg albumin denaturation assay. Based on present finding future direction also planned to conform the activity by using well established in-vivo methods.

Effect of gloriosa superba in combination with fluconazole on anti-Candida activity against biofilm development and mature biofilm growth patterns
Somwanshi Sachin M, Dhawale Shashikant C, Zore Gajanan B, Choudhary Harshad W
2TMR Integrative Medicine. 2019, 3: e19011.   https://doi.org/10.12032/TMRIM201903011
Abstract ( 70 )   HTML ( 1 )     PDF (1119KB) ( 26 )  

Highlights:

Use of G. Superba L. alone or in combination with available antifungal drugs would be a novel approach against drug resistant biofilms of C. albicans. In future it will be useful to avoid side effects associated with high dosages and long term usage of the conventional antifungal drugs during anti- biofilm therapy.

Abstract

This study was to evaluate the efficacy of methanolic extract of G. superba L in combination with Fluconazole against biofilm development and mature biofilms of C. albicans. Synergism between MEGS and Fluconazole combination against biofilm formation was evident with FICI of 0.187. Combination of MEGS and Fluconazole did not have synergistic potential against mature biofilm growth, evidenced in FICI of 0.916. MIC of standard Fluconazole was found to be 0.5 and >0.5 in biofilm development and mature biofilm respectively.

Non-pharmacological therapy of TCM for the treatment of essential hypertension
Meng-Lei Li, Shan-Fu Chen, Ying-Qiang Zhao
2TMR Integrative Medicine. 2019, 3: e19010.   https://doi.org/10.12032/TMRIM201903010
Abstract ( 225 )   HTML ( 2 )     PDF (530KB) ( 172 )  

Highlights:

It is necessary to strengthen the research on the mechanism of non-drug therapy of TCM, and combine clinical observation and experimental research to promote the promotion and application of non-pharmacological therapy of TCM in clinical practice.

Abstract

Non-pharmacological treatment is an important means to control hypertension, with many advantages such as low cost, less medication, stable blood pressure, less complications, and improved prognosis. This article reviews the research progress of non-drug treatment of hypertension in Chinese medicine in the past five years, and evaluates traditional Chinese medicine such as TCM Qigong, Wuhuo opera, Ba Duanjin and Taijiquan, and Chinese medicine acupuncture, moxibustion, massage, application, acupoint embedding and other traditional Chinese medicine. The effect of external treatment in hypertension control provides guidance for non-drug therapy to control hypertension.

A review of the treatment of ankylosing spondylitis from liver and kidney
Teng Huang, Ling-Jing Zhang, Li-Na Zhang, Zhi-Yong Zhao, Bin Hao
2TMR Integrative Medicine. 2019, 3: e19009.   https://doi.org/10.12032/TMRIM201903009
Abstract ( 64 )   HTML ( 0 )     PDF (430KB) ( 28 )  

Highlights:

On the basis of tonifying liver and kidney, the clinical symptoms of Ankylosing spondylitis can be improved by promoting blood circulation, removing blood stasis and eliminating pathogenic factors.

Abstract

Ankylosing spondylitis is a kind of arthropathy of rheumatic immune disease. The pathogenesis of ankylosing spondylitis is not very clear in current medical research. Because of the recurrence of the disease and the prolongation of the disease, the quality of life of the patients has been seriously affected. Traditional Chinese medicine has long known its etiology and pathogenesis, and it is very effective in clinical treatment. Based on the theory of traditional Chinese medicine and modern physicians' understanding of its etiology and pathogenesis, and starting from the function and characteristics of liver and kidney, this paper explores the basis of treating ankylosing spondylitis from liver and kidney, with a view to providing new ideas for the clinical treatment of ankylosing spondylitis.

The review of clinical application of X-shaped balance acupuncture therapy for joint pain and activity disorder
Cheng-Li Sun, Wen Lu, Li-Qun He, Jian-Dong Gao, Jing Xiao
2TMR Integrative Medicine. 2019, 3: e19008.   https://doi.org/10.12032/TMRIM201903008
Abstract ( 104 )   HTML ( 0 )     PDF (453KB) ( 25 )  

Highlights:

We have applied X-shaped balance acupuncture therapy in the clinic to treat local pain such as neck, shoulder, back and leg pain, and achieved good results. Most pain disorders are effective and lasting.

Abstract

Musculoskeletal diseases such as neck, shoulder, back and leg pain are extremely common in clinic. Diseases of the joints of the extremities, especially sputum, wrist sprains, and periarthritis of the shoulders, and even lumbar disc herniation, use X-shaped balance acupuncture therapy to relieve pain and improve motor function. The method of obtaining acupoints is simple and easy to operate. Many of clinical comparison shows that this therapy is simple to conventional acupuncture and moxibustion in the treatment of local pain and joint diseases. This review concluded the advantaged about the X-shaped balance acupuncture therapy and it worth to clinical promotion.

Banxiashengjiangdaizheshi decoction combined with warm acupuncture in the treatment of 10 cases of postoperative gastroparesis of gastric cancer
Gang Ye
2TMR Integrative Medicine. 2019, 3: e19007.   https://doi.org/10.12032/TMRIM201903007
Abstract ( 73 )   HTML ( 1 )     PDF (393KB) ( 14 )  

Highlights:

Banxiashengjiangdaizheshi decoction combined warm acupuncture have the function of invigorating stomach and removing turbid, warming yang and lowering reverse.

Abstract

Objective: To study the treatment of postoperative gastroparesis in patients with gastric cancer.Methods 18 patients with gastroparesis after gastric cancer surgery were divided into two groups, including 10 patients in the observation group and 8 patients in the control group. The observation group was treated with traditional Chinese medicine combined with warm acupuncture and moxibustion, and the control group was treated with western medicine.Results After 7 days treatment, there was significant difference in cure rate and total effective rate between the two groups (P < 0.01), and the treatment group was obviously superior to the control group.Conclusion Traditional Chinese medicine combined with warm acupuncture is effective in the treatment of postoperative gastroparesis of gastric cancer.

Exploring the relationship between Ca2+ and the "Qi" of TCM from the perspective of modern medicine
Ai-Wen Zhuang, Xue-Min Fan, Li-Na Ji, Xian Deng, Hong Ling, Rong-Qun Li
2TMR Integrative Medicine. 2019, 3: e19006.   https://doi.org/10.12032/TMRIM201903006
Abstract ( 162 )   HTML ( 1 )     PDF (525KB) ( 82 )  

Highlights:

There are many similarities and close relationships between Qi and Ca2+ in terms of source and function.

Abstract

Ancient Chinese philosophy believed that Qi is the origin of all things in the universe, and it is the extremely fine material that exists in the universe and is ubiquitous. The Qi of traditional Chinese medicine (TCM) is put forward on the basis of philosophical meaning. It points out that Qi is a very invasive and extremely subtle substance with strong vitality in the human body. It is the basic substance that constitutes the human body and maintains its life activity. Qi is the basic concept of TCM, but the understanding of the essence of Qi is not clear. At present, there are more and more studies on the essence of Qi of TCM, but it has not achieved a major breakthrough, which has become one of the problems that limit the development of traditional Chinese medicine. Through the research on the literature, it is found that Qi and Ca2+ are inextricably similar. This paper attempts to explore the relationship between Qi and Ca2+ from the aspects of source of production and physiological function to explain their similar points. We hope to provide new ideas for the study of the essence of Qi and achieve the purpose of promoting the development of TCM.

Chinese herbal protocol anti-fibrosis 1 improves radiation induced fibrosis and its related dysfunctions
Dong-Mei Jiang, Ying-Ying Jin, Liu-Hua Long, Li-Jue Zhang, Si-Hai Tan, Pei-Pei Song, Long Zhang, Tai Zhang, Wen-Hua Zhang, Feng-Wei Wang
2TMR Integrative Medicine. 2019, 3: e19005.   https://doi.org/10.12032/TMRIM201903005
Abstract ( 87 )   HTML ( 0 )     PDF (546KB) ( 15 )  

Highlights:

In this study, TCM was used to treat the local fibrosis and the corresponding dysfunction was significantly improved. It shows that TCM has a good prospect in the treatment of radiation injury.

Abstract

Objective: Radiotherapy is the main modality for treatment of malignancies, but radiation fibrosis is a severe side effect of radiotherapy which may cause severe dysfunction of patients. We design a new traditional Chinese herbal protocol, which is named as Anti-fibrosis 1, and use it in patients with severe radiation fibrosis. Methods: From Feb 2013 to Feb 2014, 23 patients with severe radiation fibrosis and apparent dysfunction were treated with anti-fibrosis 1. There were 7 patients with nasopharyngeal carcinoma, 7 with oral cavity and oral pharyngeal carcinoma, 2 with pharyngeal carcinoma, 2 with breast carcinoma, 2 with esophageal carcinoma and 3 patients with pelvic malignancies. All patients received radiotherapy at least 0.5-8 years ago, and 22 patients with complete response, but all patients suffered with severe radiation fibrosis. The radiation dosage was among 50-74Gy/25-37f/35-70d. Patients status of fibrosis and recovery of tissue and organ were evaluated after 0.5, 1.5 and 3 months of herbal treatment. Results: 22 of 23 patients were head and neck carcinoma, 20 patients had got partial relief after 15 days medication with anti-fibrosis 1, nearly all patients had got apparent relief after 1.5 month of medication. But, there was no apparent difference between 1.5 month and 3 months group. There were no apparent side effects after the herbal treatment. Conclusions: The traditional Chinese herbal protocol anti-fibrosis 1 is an effective and safe modality for radiation fibrosis. And the apparent effect occurred after 1.5 month of herbal medication.

Pathogenesis and treatment status of adenomyosis complicated with infertility
Hua Wang, Chun-Hua Cao, Yan Chen, Saili Wang, Qin Wang
2TMR Integrative Medicine. 2019, 3: e19004.   https://doi.org/10.12032/TMRIM201903004
Abstract ( 78 )   HTML ( 0 )     PDF (426KB) ( 10 )  

Highlights:

The treatment options for patients of AD with infertility should follow the principle of individual treatment and choose the most appropriate method based on the specific conditions of the hospital.

Abstract

Adenomyosis (AD) refers to the endometrium (including glands and stroma) into myometrium growth of pathological changes, with the delay of women's childbearing age, the patients with AD-associated infertility increased significantly. Although scholars disagreed about the effect of AD on infertility, more and more evidence showed that AD was closely related to the structural and functional defects of the eutopic endometrium and myometrial junctional zone (MJZ), which may lead to embryo implantation failure and infertility. With the rapid development of imaging technology, the accuracy of diagnose AD has been enhanced, more and more infertility patients were diagnosed with AD. At the same time, a series of pathogenic hypothesis have been proposed to explain the relationship between AD. In addition, AD may influence the patient’s reproductive function by affecting sperm transport, embryo implantation, altering uterine peristalsis, influencing the structure and function of endometrium, and changing the intrauterine environment. At present, the treatment of AD-associated infertility is similar to the treatment of AD. The commonly used treatment methods include drug therapy, conservative operative treatment, drug combination surgery, and Chinese medicine treatment and so on. The clinical and experimental studies of AD complicated with infertility and its possible pathogenesis and treatment in recent years are reviewed in this article.

Research progress of traditional Chinese medicine nursing for pelvic inflammatory disease sequelae
Pei-Yao Li, Wen-Wen Zhao, Chun-Hua Zhang, Ling Wang, Hui-Xin Ding, Chun-Guang Yu
2TMR Integrative Medicine. 2019, 3: e19003.   https://doi.org/10.12032/TMRIM201903003
Abstract ( 101 )   HTML ( 0 )     PDF (387KB) ( 10 )  

Highlights:

The knowledge of TCM syndrome differentiation of clinical staff should be strengthened to lay a foundation for clinical nursing, so as to provide individualized education programs.

Abstract

Pelvic inflammatory disease refers to a group of female genital tract on infectious disease, mainly including endometritis, tubal phlogistic, oviduct ovarian abscess, if not timely treatment of the disease is easy to become chronic, now called pelvic inflammatory disease sequelae, clinical manifestations in chronic pelvic pain, recurrent pelvic inflammatory disease, affect women's physical and mental health. TCM nursing based on the basic theory of TCM, apply the overall view, the nursing method of syndrome differentiation, the traditional Chinese medicine nursing technique is widely used in the disease, such as traditional Chinese medicine enema, Chinese medicine external treatment, moxibustion, ear acupuncture point buries a seed, methods of modern nursing, has the characteristics of simple and cheap effect, can reduce the swelling fights, promoting blood circulation to remove blood stasis, relieve the pelvic congestion, promote drainage in abdominal and pelvic cavity and the absorption of bag piece, adjust the viscera function, obvious effect and has unique advantages, further prove the uniqueness of TCM nursing and safety, provide the basis for clinical treatment.

Effect of the Tiaobufeishen decoction on Caveolin-1-p38 MAPK signaling pathway and mechanism of improving the tracheobronchomalacia in chronic obstructive pulmonary disease
Peng-Cheng Zhou, Wei Yu, Ke-Ling Chen, Wen-Jun Tang, Wei Xiao, Qian-Ming Xia, Jun-Mei Ma, Yan Dong
2TMR Integrative Medicine. 2019, 3: e19002.   https://doi.org/10.12032/TMRIM201903002
Abstract ( 128 )   HTML ( 2 )     PDF (1147KB) ( 33 )  

Highlights:

Tiaobufeishen decoction could decrease the expression of the caveolin-1, p-p38, MMP3, inhibit the activation of the caveolin-1-p38MAPK signaling pathway.

Abstract

Objective: Reliving the relationship of the Caveolin-1-p38 MAPK signaling pathway and COPD tracheobronchomalacia, and research the mechanism of Tiaobufeishen decoction improve the regression of the weasand cartilage cells.Methods Flow cytometry was used to analyze the apoptosis rate to determine the optimal concentration of Tiaobufeishen decoction and CSE, CCK8 assay was used to determine the optimal concentration of P38-MAPK specific inhibitor. The COPD cell model was created by tracheal chondrocyte which dispose by optimal concentration CSE, then add the IL-1β set up the chondrocyte degeneration model, use the method of toluidine blue staining and immunohistochemical authenticate degeneration of cartilage. This research included control group, model group, model-Tiaobufeishen group, model-blocker group. When the model was set up succeed, add the Tiaobufeishen decoction and P38-MAPK blocker in the model-Tiaobufeishen and model-blocker groups, respectively. Western Blot was used to detect the expression of caveolin-1 and p-p38 in the chondrocyte. RT-PCR was used to detect the expression of MMP3 and caveolin-1 in the matrix.Results The cell activity was not influence by the concentration of Tiaobufeishen decoction and blocker, the concentration of the CSE model was moderation. Compared with control group, the level of caveolin-1, p38MAPK, MMP3 in the model group was significant increase, moreover, the result of toluidine blue staining and immunohistochemical methods show that the chondrocyte has obvious regression. The expression of caveolin-1, p38MAPK, and MMP3 have significant decrease than the control group, and the reduction of chondrocyte degeneration.Conclusion The caveolin-1-p38MAPK signaling pathway play an important role in the morbidity of the tracheobronchomalacia. Tiaobufeishen decoction could decrease the expression of the caveolin-1, p-p38, MMP3, inhibit the activation of the caveolin-1-p38MAPK signaling pathway, therefore, it can improve the tracheobronchomalacia.

A method for isolating rat cardiomyocytes by acute enzymatic hydrolysis
Bo Liang, Hui-Ling Liao
2TMR Integrative Medicine. 2019, 3: e19001.   https://doi.org/10.12032/TMRIM201903001
Abstract ( 172 )   HTML ( 2 )     PDF (1152KB) ( 95 )  

Highlights:

This research was introduced a method of isolate rat cardiomyocytes though acute enzymatic hydrolysis, provides a new direction for clinical research.

Abstract

Objective: This research showed a method of isolate rat cardiomyocytes though acute enzymatic hydrolysis. Method: Our method was divided into five steps: preparation, heart extraction, perfusion, mechanical dissociation and purification and recalcification. Firstly, make a preparation, including solutions, equipment and so on. Secondly, after anesthesia satisfactorily, open the chest of rat and take the heart out. Thirdly, transect the aorta and hang the aorta onto the Langendroff perfusion system such that the tip is just distal to the aortic valve. Forth, perfuse the heart with digestion solution for 17-20 min after Ca2+ free tyrode solution for 5 min. Finally, remove the heart from the system quickly and dissociate mechanically in the KB solution and recalcification after filter. Result: Though the microscope, good-state cardiomyocytes (clear stripes, strong sense of three-dimensional, stick well) can be observed. Conclusion: Those cells can be used to undergo medical electrophysiological experiment, including the study of iron channel.

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