Objective: To establish a simple, effective and high-purity primary culture method for fetal rat hippocampal neurons. Methods: Wistar rats of gestational age 18 days were taken and the brain tissue was separated under the microscope. Single neuronal cells were obtained by digestion with Brain Dissociation Kit, and then were seeded in cell plates to observe the basic morphologic structure after 24h, 3d, and 5d. Immunofluorescence of microtubule associated protein 2 was applied to assess cell purity of the culture. Results: The hippocampal neurons obtained in this culture method are in good condition and grow vigorously. On the 7th day after culture, the purity of neurons was up to 99.62%. Conclusion: The method is simple and effective for obtaining the high-purity and stable neurons.
The application of blood-letting puncture and cupping in the treatment of scapulohumeral periarthritis can provide strong evidence for future clinical treatment.
Objective: To systematically evaluate the clinical efficacy and safety of blood-letting puncture and cupping in the treatment of periarthritis of shoulder. Methods: We electronically searched databases including CNKI, VIP, PubMed, WanFang Data, EMbase Cochrane Library and to collect randomized controlled trials (RCTs) and quasi-randomized controlled trials about blood-letting puncture and cupping for periarthritis of shoulder and evaluated the quality of the documents collected by the Cochrane collaboration, provided by Review Manager 5 software for statistical analysis. Then the quality of the included trials was assessed using the Jadad scale and GRADE system was used to score the quality and grade recommendation. Results: Thirty RCTs involving 2556 patients were included. The results of meta-analysis showed that: Total effective rate OR = 6.21 [OR = 6.21, 95％CI (4.69, 8.21), P < 0.001]. The results of GRADE system evaluation showed that the level of evidence was B, and the recommendation level was weak recommendation. Conclusion: Blood-letting puncture and cupping shows better effects than other treatments and preferably security in the treatment of periarthritis of shoulder. We do not find any serious adverse reaction reports. Due to limited quality of the included studies, more high quality studies are needed to verify the above conclusion.
This review reveals practical implications and future research directions, benefiting the development of acupuncture in the modern medical arena for both patients and practitioners.
Being overweight and obese impacts health due to adiposity itself and illnesses it incurs, which produces individual and societal financial burdens. This urges effective interventions, including adjunct therapies. Acupuncture, a traditional Chinese medical practice, indicates positive signs for tackling these problems. Investigating 101 empirical human-based projects, this narrative review analyses their outcomes and then provides an overview of the effects of acupuncture in body weight reduction and obesity-induced diseases, either performed alone or with other approaches. It involves 15941 participants from 9-80 years old in mainland China, Taiwan, Turkey, Brazil, Egypt, Iran, Greece, Korea, Sudan, Thailand, and the USA. It also reveals practical implications and future research directions, benefiting the development of acupuncture in the modern medical arena for both patients and practitioners.
The practical randomized controlled trial reveal that the COPD patients will be benefit from therapy with Traditional Chinese medicine treatment.
Background: The prevalence, morbidity and mortality of chronic obstructive pulmonary disease (COPD) is very high, so it has become a public health problem. The early stage of COPD has been paid much attention before it develops severely. As well, traditional Chinese medicine has some advantages in relieving symptoms and reducing the incidence of COPD exacerbations. Methods: A practical randomized controlled trial will be designed to test the clinical effects of traditional Chinese medicine treatment according to syndrome differentiation. A total of 80 patients with mild / moderate COPD will participate in this study, 40 in the treatment group and 40 in the control group. On the basis of the lifestyle intervention, traditional Chinese medicine formula granules for treatment group and the placebo simulation of traditional Chinese medicine granules for control group. The exacerbation and the improvement of pulmonary function will be used as the primary outcome measures. The improvement of clinical symptoms and signs, the quality of life and Dyspnea will be used as the secondary outcomes measures. Conclusion: We assume that the COPD patients will be benefit from therapy with Traditional Chinese medicine treatment.
The results of this experiment shows that the continuous sodium current increases under anoxic environment.
The aim of this study was to investigate the effect of hypoxia on the sodium current of rat cardiomyocytes in order to explore ion channel mechanism of cardiomyocyte hypoxia. The rat cardiomyocytes were isolated by acute enzymatic hydrolysis. A group of untreated cells were used to record sodium currents using whole-cell patch-clamp technique, another group was subjected to hypoxia and record sodium currents using same technique. The results showed that the morphological trajectory of sodium hypoxia was not changed compared with that of normal cells. The I-V curve of hypoxic cells was significantly higher than that of normal cells, and the peak current of INa was 15.68% higher than that of normal cells (P < 0.0001). Activation potential of normal and hypoxia cells was about -40mV, the maximum peak current corresponds to the stimulation voltage of -25mV. The above results suggest that rat cardiomyocytes sodium current increases in the case of hypoxia.
This research was introduced a method of isolate rat cardiomyocytes though acute enzymatic hydrolysis, provides a new direction for clinical research.
Objective: This research showed a method of isolate rat cardiomyocytes though acute enzymatic hydrolysis. Method: Our method was divided into five steps: preparation, heart extraction, perfusion, mechanical dissociation and purification and recalcification. Firstly, make a preparation, including solutions, equipment and so on. Secondly, after anesthesia satisfactorily, open the chest of rat and take the heart out. Thirdly, transect the aorta and hang the aorta onto the Langendroff perfusion system such that the tip is just distal to the aortic valve. Forth, perfuse the heart with digestion solution for 17-20 min after Ca2+ free tyrode solution for 5 min. Finally, remove the heart from the system quickly and dissociate mechanically in the KB solution and recalcification after filter. Result: Though the microscope, good-state cardiomyocytes (clear stripes, strong sense of three-dimensional, stick well) can be observed. Conclusion: Those cells can be used to undergo medical electrophysiological experiment, including the study of iron channel.
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