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Clinical studies have been used to evaluate the safety and efficacy of potentially effective for disease therapies
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Analyse the rules of Chinese Herbal of Professor Xiong Lu in treating Metaphase and Advanced lung cancer by data mining
Dao-Rui Hou, Li Zhu, Ya-Hui Peng, Lu Xiong
TMR Integrative Medicine    2017, 1 (2): 37-44.   https://doi.org/10.12032/TMRIM201701115
Abstract PDF (668KB)  
Objective: To analyze the experience of chief physician Xiong Lu in treating Metaphase and Advanced lung cancer through using TCM inheritance support system (V2.5 ). Method: Collecting the prescriptions used for Metaphase and Advanced lung cancer from November 1, 2014 to February1, 2015,  then the data were entered into the TCM inheritance support system. Based on principle analysis, revised mutual information, complex system entropy cluster and unsupervised hierarchical clustering composing principles were analyzed. Result: Based on the analysis of 228 case of prescriptions, the frequency of each Chinese medicinal herb and association rules among herbs included in the database were computed, 15 core combinations and 2 new prescriptions were explored from the database. Conclusion: In treating Metaphase and Advanced lung cancer, chief physician Xiong Lu pay attention to Fuzheng Peiben (Therapy for Support Zheng-qi to Propup Root), according to the different situation cooperate with Tong Luo (dredging collaterals), San Jie (Dissipating a Mass), Huo Xue (Activating blood), Gong Du (Counteracting Toxic Substance) and so on. Xiong Lu is also good at using toxic drugs and incompatible medicaments. chief physician Xiong Lu's unique experience is worthy of further mining.
An improved primary culture method for hippocampal neurons in fetal rats and MAP2 identification
Yi-Ran Shao, Yong Chang DI WU, Tao Yue
2TMR Integrative Medicine    2018, 2 (1): 1-6.   https://doi.org/10.12032/TMRIM201802011
Abstract HTML PDF (359KB)  

Objective: To establish a simple, effective and high-purity primary culture method for fetal rat hippocampal neurons. Methods: Wistar rats of gestational age 18 days were taken and the brain tissue was separated under the microscope. Single neuronal cells were obtained by digestion with Brain Dissociation Kit, and then were seeded in cell plates to observe the basic morphologic structure after 24h, 3d, and 5d. Immunofluorescence of microtubule associated protein 2 was applied to assess cell purity of the culture. Results: The hippocampal neurons obtained in this culture method are in good condition and grow vigorously. On the 7th day after culture, the purity of neurons was up to 99.62%. Conclusion: The method is simple and effective for obtaining the high-purity and stable neurons.

Analysis of 610 cases inpatients with chronic heart failure
Bo-Yong Qiu, Yong-Xia Wang, Zuo-Ying Xing, Bin Li, Ming-Jun Zhu, Peng Chen, Jia Zheng
TMR Integrative Medicine    2018, 2 (2): 87-91.   https://doi.org/10.12032/TMRIM201802025
Abstract PDF (237KB)  
Objective: To analyze the clinical characteristics and medical treatment of hospitalized patients with chronic heart failure, and provide medication for the patients. Methods: According to medical records of inpatients, demographic information, etiology, clinical features and treatment information were collected. Results: A total of 610 cases with heart failure from our hospital between July 2010 and June 2016 were analyzed. The average age of all the patients was 63. Males accounted for 50.49%.There were 82.7% patients with NYHA functional classification at III-IV. 31.3% patients with coronary artery disease, 28.4% with rheumatic heart diseases and 21.8% with expansion of heart disease. 27.4 percent of the patients with the left ventricular ejection fraction lower than 40%.There were 78.9% patients received aldosterone antagonist treatment, 63.4% received ACEI / ARB, 62.1% received digoxin, 59.8% received beta blockers, and 75.4% patients received decoction, 80.8% received Chinese patent medicine. Conclusion: The study have shown that high utilization of Traditional Chinese medicine is to highlight the advantages of the combination of Traditional Chinese and Western medicine in the treatment of heart failure. The usage of spironolactone, ACEI / ARB, beta blockers in our hospital was lower than the guideline ratio, higher than that of the national average. However, the use of diureticis is lower than that of national average, which may relate to the use of damp-clearing herbs. Clinicians should pay attention to the high utilization rate of digoxin. Patients with the left ventricular ejection fraction > 50% accounted for 61.1%, which required more attention should be paied to the diagnosis and treatment of patients with heart failure of the ejection fraction remains.
Progress in the study of diabetic retinopathy
Lei Xiao, Jun-Guo Duan
TMR Integrative Medicine    2018, 2 (2): 62-67.   https://doi.org/10.12032/TMRIM201802022
Abstract PDF (247KB)  
Diabetic retinopathy (DR) is one of the microvascular complications of diabetes, which could be divided into non-proliferative DR and proliferative DR according to the severity of retinopathy. Diabetic microangiopathy not only affects the eyesight of patients with diabetes, but also endanger the lives of patients and draws more and more attention from domestic and foreign scholars. The etiology and treatment of diabetes are reviewed to explore ways of treating diabetic retinopathy.
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Efficacy of blood-letting puncture and cupping in the treatment of periarthritis of shoulder: a systematic review
Li-Hui Zhang, Li-Xin Wang, Qian Cui, Guo-Wei Zhang
2TMR Integrative Medicine    2018, 2 (4): 175-191.   https://doi.org/10.12032/TMRIM201802033
Abstract HTML PDF (1123KB)  

Highlights:

The application of blood-letting puncture and cupping in the treatment of scapulohumeral periarthritis can provide strong evidence for future clinical treatment.

Abstract

Objective: To systematically evaluate the clinical efficacy and safety of blood-letting puncture and cupping in the treatment of periarthritis of shoulder. Methods: We electronically searched databases including CNKI, VIP, PubMed, WanFang Data, EMbase Cochrane Library and to collect randomized controlled trials (RCTs) and quasi-randomized controlled trials about blood-letting puncture and cupping for periarthritis of shoulder and evaluated the quality of the documents collected by the Cochrane collaboration, provided by Review Manager 5 software for statistical analysis. Then the quality of the included trials was assessed using the Jadad scale and GRADE system was used to score the quality and grade recommendation. Results: Thirty RCTs involving 2556 patients were included. The results of meta-analysis showed that: Total effective rate OR = 6.21 [OR = 6.21, 95%CI (4.69, 8.21), P < 0.001]. The results of GRADE system evaluation showed that the level of evidence was B, and the recommendation level was weak recommendation. Conclusion: Blood-letting puncture and cupping shows better effects than other treatments and preferably security in the treatment of periarthritis of shoulder. We do not find any serious adverse reaction reports. Due to limited quality of the included studies, more high quality studies are needed to verify the above conclusion.

Efficacy of acupuncture on treating obesity and adipose-incurred illnesses
Fung-Kei Cheng
2TMR Integrative Medicine    2018, 2 (4): 140-167.   https://doi.org/10.12032/TMRIM201802031
Abstract HTML PDF (1399KB)  

Highlights:

This review reveals practical implications and future research directions, benefiting the development of acupuncture in the modern medical arena for both patients and practitioners.

Abstract

Being overweight and obese impacts health due to adiposity itself and illnesses it incurs, which produces individual and societal financial burdens. This urges effective interventions, including adjunct therapies. Acupuncture, a traditional Chinese medical practice, indicates positive signs for tackling these problems. Investigating 101 empirical human-based projects, this narrative review analyses their outcomes and then provides an overview of the effects of acupuncture in body weight reduction and obesity-induced diseases, either performed alone or with other approaches. It involves 15941 participants from 9-80 years old in mainland China, Taiwan, Turkey, Brazil, Egypt, Iran, Greece, Korea, Sudan, Thailand, and the USA. It also reveals practical implications and future research directions, benefiting the development of acupuncture in the modern medical arena for both patients and practitioners.

Based on the process management of traditional Chinese medicine comprehensive intervention method on outcomes in patients with mild / moderate chronic obstructive pulmonary disease: study protocol for a practical randomized controlled trial
Ming Ren, Li-Ping Guo, Xiao Sun, Yang Qiao, Lei Chen, Lei Zhang, Yan Shao, Xiang-Li Han, Huai-Ying Ding, Jian-Mei Zhao
2TMR Integrative Medicine    2018, 2 (4): 197-201.   https://doi.org/10.12032/TMRIM201802035
Abstract HTML PDF (593KB)  

Highlights:

The practical randomized controlled trial reveal that the COPD patients will be benefit from therapy with Traditional Chinese medicine treatment.

Abstract

Background: The prevalence, morbidity and mortality of chronic obstructive pulmonary disease (COPD) is very high, so it has become a public health problem. The early stage of COPD has been paid much attention before it develops severely. As well, traditional Chinese medicine has some advantages in relieving symptoms and reducing the incidence of COPD exacerbations. Methods: A practical randomized controlled trial will be designed to test the clinical effects of traditional Chinese medicine treatment according to syndrome differentiation. A total of 80 patients with mild / moderate COPD will participate in this study, 40 in the treatment group and 40 in the control group. On the basis of the lifestyle intervention, traditional Chinese medicine formula granules for treatment group and the placebo simulation of traditional Chinese medicine granules for control group. The exacerbation and the improvement of pulmonary function will be used as the primary outcome measures. The improvement of clinical symptoms and signs, the quality of life and Dyspnea will be used as the secondary outcomes measures. Conclusion: We assume that the COPD patients will be benefit from therapy with Traditional Chinese medicine treatment.

Effects of hypoxia on sodium current in rat cardiomyocytes
Bo Liang, Hui-Ling Liao
2TMR Integrative Medicine    2018, 2 (4): 192-196.   https://doi.org/10.12032/TMRIM201802034
Abstract HTML PDF (668KB)  

Highlights:

The results of this experiment shows that the continuous sodium current increases under anoxic environment.

Abstract

The aim of this study was to investigate the effect of hypoxia on the sodium current of rat cardiomyocytes in order to explore ion channel mechanism of cardiomyocyte hypoxia. The rat cardiomyocytes were isolated by acute enzymatic hydrolysis. A group of untreated cells were used to record sodium currents using whole-cell patch-clamp technique, another group was subjected to hypoxia and record sodium currents using same technique. The results showed that the morphological trajectory of sodium hypoxia was not changed compared with that of normal cells. The I-V curve of hypoxic cells was significantly higher than that of normal cells, and the peak current of INa was 15.68% higher than that of normal cells (P < 0.0001). Activation potential of normal and hypoxia cells was about -40mV, the maximum peak current corresponds to the stimulation voltage of -25mV. The above results suggest that rat cardiomyocytes sodium current increases in the case of hypoxia.

A method for isolating rat cardiomyocytes by acute enzymatic hydrolysis
Bo Liang, Hui-Ling Liao
2TMR Integrative Medicine    2019, 3: e19001.   https://doi.org/10.12032/TMRIM201903001
Abstract HTML PDF (1152KB)  

Highlights:

This research was introduced a method of isolate rat cardiomyocytes though acute enzymatic hydrolysis, provides a new direction for clinical research.

Abstract

Objective: This research showed a method of isolate rat cardiomyocytes though acute enzymatic hydrolysis. Method: Our method was divided into five steps: preparation, heart extraction, perfusion, mechanical dissociation and purification and recalcification. Firstly, make a preparation, including solutions, equipment and so on. Secondly, after anesthesia satisfactorily, open the chest of rat and take the heart out. Thirdly, transect the aorta and hang the aorta onto the Langendroff perfusion system such that the tip is just distal to the aortic valve. Forth, perfuse the heart with digestion solution for 17-20 min after Ca2+ free tyrode solution for 5 min. Finally, remove the heart from the system quickly and dissociate mechanically in the KB solution and recalcification after filter. Result: Though the microscope, good-state cardiomyocytes (clear stripes, strong sense of three-dimensional, stick well) can be observed. Conclusion: Those cells can be used to undergo medical electrophysiological experiment, including the study of iron channel.

Advances in the research of high myopia treatment
Ming-shan Liu, Ya-Mo
2TMR Integrative Medicine    2017, 1 (3): 124-129.   https://doi.org/10.12032/TMRIM201701124
Abstract PDF (236KB)  
High myopia, is a condition of the diopter over -6.00D, accompanied by axial extension and fundus changes, such as temporal arc plaque, pigment epithelium thinning, leopard-shaped fundus, fuchs spot, retina choroidal atrophy, progressive decline in vision, as well as amblyopia, glaucoma, cataract, vitreous opacity, retinal detachment and other ophthalmic diseases. High myopia is one of the common causes of blindness in China. It is necessary to review these cases in order to better guide the clinical and improve the level of vision of patients.
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