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The core is to study the influence of traditional Chinese medicine and modern medicine on diseases
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Effect of Xuebijing injection on hematopoietic homeostasis of LPS induced sepsis in mice
Ping Wang, Jun Wang, Ying Hao, Xiao-Rui Li, Ya-Ru Feng, Lan Wang, Hong-Cai Shang, Jian-Xun Wang
6TMR Modern Herbal Medicine    2019, 2 (4): 206-214.   https://doi.org/10.12032/TMRmhm2017A60
Abstract PDF (1088KB)  
Objective: To explore the effect and mechanism of Xuebijing injection (XBJ) on the hematopoietic homeostasis of bone marrow (BM) in septic mice. Methods: BM cells stimulated with XBJ were analyzed by inverted optical microscope and qPCR, to evaluate the effect of XBJ on differentiation function of BM cells in vitro. Lipopolysaccharide (LPS) 055:B5 at the dose of 20mg/kg was used to establish the sepsis model. To determine the hematopoietic stem cells homeostasis affected by LPS in mice, BM cells were isolated and analyzed by flow cytometry based on the immunophenotypic surface markers. 20ml/kg of XBJ was administered by tail vein once/day after peritoneal injection of LPS. All animals were sacrificed on the fifth day. The frequency of hematopoietic stem cells (HSC) and immune cells in mice were quantified by flow cytometry. The gene expression of transcription factors were detected by qPCR. Results: XBJ promoted myeloid differentiation of BM cells in vitro, which may be related to the mRNA expression of the transcription factors. The results in vivo showed the percentage of BM Lin-SCA-1+c-KIT+ (LSK) cells, and Long-term HSCs (LT-HSC) were significantly increased in LPS group. But the percentage of multipotential progenitors (MPPs), granulocyte-monocyte progenitor (GMP) and megakaryocytic-erythroid precursor (MEP) were significantly decreased in LPS group. Whereas, XBJ improved the immune function in sepsis mice by suppression the LSK expansion in vivo. The result of qPCR showed that LSD1 and PU.1 mRNA expression in XBJ group were significantly higher than LPS group and control group respectively. Conclusion: Xuebijing injection can improve immune function in sepsis mice by regulating hematopoietic homeostasis. Its mechanism may be related to the up-regulation of LSD1 and PU.1 gene expressions.
Network pharmacology based method for mechanistic investigation of the Compound Xintahua in the treatment of atherosclerosis
Yu-Che Wu, Yan-Ming Wang, Na-Bi Xinhua
6TMR Modern Herbal Medicine    2019, 2 (4): 225-236.   https://doi.org/10.12032/TMRmhm2017B57
Abstract PDF (683KB)  
Objective: To explore the pharmacological basis of the Compound Xintahua (XTH) action in Atherosclerosis (AS) therapy, a network interaction analysis was conducted at the molecular level. Methods: TCMSP database and literature mining were used to analyze the main effective components in XTH, and the targets were predicted by Swiss Target Prediction server according to AS mechanism. The potential targets were introduced into the FunRich database for target annotation and analysis, the path analysis was finally performed based on the FunRich databases. To determine the mechanism of action of XTH. Results: A total of 316 compounds, 117 targets, and 290 signaling pathways were identified. And 16 effective compounds, 39 common targets, and 43 pathways were associated with AS. Conclusions: The results showed that the flavonoids, phenols, organic acids and terpenoids of XTH could participate in the process of lipid metabolism, angiogenesis, oxidation, inflammation, endocrine metabolism, cell proliferation and apoptosis, It was further found that they could play the role of anti-Atherosclerosis through multi-component, multi-target, and multi-channel synergistically.
Compatible stability study of XingNaoJing injection based on physical-chemical properties analysis
Dong-Li Qi, Chun-Xia Liang, Yue-Lin Zhang, Yan-Quan Gao, Yue Xing, Hong-Li Yan, Bing Zhang, Jia-Rong Xie, Zhi-Dong Liu
6TMR Modern Herbal Medicine    2019, 2 (4): 173-182.   https://doi.org/10.12032/TMRmhm2017A55
Abstract PDF (1140KB)  
Objective: The clinical treatment of brain diseases is urgent. Xingnaojing (XNJ) injection is often used in combination with other injection drugs. Due to the possible interaction between the injections in vivo, the particle size, osmotic pressure, pH value change and component stability decrease, that is one of the important factors causing various adverse reactions. Based on the above situation, this study investigated the physical properties and chemical composition changes of XNJ injection and its compatibility solvent and 13 kinds of clinical injection, speculated the possible interactions between the drugs in vivo from the perspective of in vitro compatibility stability, find out the safety risks of adverse reactions and provide guidance for the safe and rational use of XNJ injection. Methods: According to the clinical application, XNJ injection was mixed with 13 combination injections based on 250 mL 5% glucose injection, and placed at room temperature for 6 h. Then, the clarity, particle size, pH, osmolality, and the contents of camphor, d-borneol, and muscone of the compatible solutions were detected at 0, 1, 2, 4, and 6 h, respectively. Results: The results showed that the physical-chemical properties of compatibility solution were slightly influenced when XNJ was combined with Alprostadil injection and Danhong injection. The change of particle size and the degradation of muscone content were the main factors affecting the compatibility stability of XNJ injection, indicating that there are some problems in compatibility stability, which may be one of the causes of clinical adverse reactions. Conclusion: This study suggests that XNJ injection in combination with other injections during intravenous administration should be performed cautiously.
The efficacy and safety of Chinese herbal medicine combined with ACEI/ARB for treatment of incipient diabetic nephropathy: A meta-analysis
Qi-Yan Zheng, Wei-Jing Liu, Wei-Wei Sun, Yao-Xian Wang, Hong-Fang Liu, Lu-Ying Sun
2TMR Integrative Medicine    2018, 2 (1): 7-29.   https://doi.org/10.12032/TMRIM201802012
Abstract HTML PDF (1365KB)  

Objective: This meta-analysis evaluated the efficacy and safety of Chinese herbal medicine (CHM) combined with angiotensin-converting enzyme inhibitors (ACEIs) and/or angiotensin-receptor blockers (ARBs)for treatment of incipient diabetic nephropathy(IDN). Methods: Nine data bases were searched for randomized controlled trials of Chinese herbal medicine(CHM) and ACEI/ARB for treatment of IDN. Included articles were published between January2006 and December 2016. All studies were divided into prescriptions containing both Astragali Radix and Rehmanniae Radix (i subgroup), Astragali Radix(Huangqi) or Rehmanniae Radix(Dihuang) (ii subgroup), neither Astragali Radix nor Rehmanniae Radix (iii subgroup). Review Manager 5.3was used for subgroup analysis. Results: In total, 28 RCTs with 2017 patients were included. The results showed 1)the urinary albumin excretion rate (UAER) can be reduced significantly using CHM with ACEI or ARB for treatment of IDN compared to ACEI or ARB alone, and reduction of the UAER of the i subgroup was superior to that of the other two subgroups;2)serum creatinine (Scr) levels can be reduced significantly using CHM combined with ACEI or ARB, and reduction of Scr in the ii subgroup was superior to that in the iii subgroup;3)reduction of BUN in group A was not better than that in group B. Conclusion: In summary, CHMs combined with ACEI/ARB can decrease UAER and Scr significantly compared to the use of ACEI/ARB during IDN treatment. The effect was more significant in the CHM group containing Astragali Radix or Rehmanniae Radix. The application of Astragali Radix and Rehmanniae Radix should be emphasized in third stage diabetic nephropathy.

Effect of the Tiaobufeishen decoction on Caveolin-1-p38 MAPK signaling pathway and mechanism of improving the tracheobronchomalacia in chronic obstructive pulmonary disease
Peng-Cheng Zhou, Wei Yu, Ke-Ling Chen, Wen-Jun Tang, Wei Xiao, Qian-Ming Xia, Jun-Mei Ma, Yan Dong
2TMR Integrative Medicine    2019, 3: e19002.   https://doi.org/10.12032/TMRIM201903002
Abstract HTML PDF (1147KB)  

Highlights:

Tiaobufeishen decoction could decrease the expression of the caveolin-1, p-p38, MMP3, inhibit the activation of the caveolin-1-p38MAPK signaling pathway.

Abstract

Objective: Reliving the relationship of the Caveolin-1-p38 MAPK signaling pathway and COPD tracheobronchomalacia, and research the mechanism of Tiaobufeishen decoction improve the regression of the weasand cartilage cells.Methods Flow cytometry was used to analyze the apoptosis rate to determine the optimal concentration of Tiaobufeishen decoction and CSE, CCK8 assay was used to determine the optimal concentration of P38-MAPK specific inhibitor. The COPD cell model was created by tracheal chondrocyte which dispose by optimal concentration CSE, then add the IL-1β set up the chondrocyte degeneration model, use the method of toluidine blue staining and immunohistochemical authenticate degeneration of cartilage. This research included control group, model group, model-Tiaobufeishen group, model-blocker group. When the model was set up succeed, add the Tiaobufeishen decoction and P38-MAPK blocker in the model-Tiaobufeishen and model-blocker groups, respectively. Western Blot was used to detect the expression of caveolin-1 and p-p38 in the chondrocyte. RT-PCR was used to detect the expression of MMP3 and caveolin-1 in the matrix.Results The cell activity was not influence by the concentration of Tiaobufeishen decoction and blocker, the concentration of the CSE model was moderation. Compared with control group, the level of caveolin-1, p38MAPK, MMP3 in the model group was significant increase, moreover, the result of toluidine blue staining and immunohistochemical methods show that the chondrocyte has obvious regression. The expression of caveolin-1, p38MAPK, and MMP3 have significant decrease than the control group, and the reduction of chondrocyte degeneration.Conclusion The caveolin-1-p38MAPK signaling pathway play an important role in the morbidity of the tracheobronchomalacia. Tiaobufeishen decoction could decrease the expression of the caveolin-1, p-p38, MMP3, inhibit the activation of the caveolin-1-p38MAPK signaling pathway, therefore, it can improve the tracheobronchomalacia.

Banxiashengjiangdaizheshi decoction combined with warm acupuncture in the treatment of 10 cases of postoperative gastroparesis of gastric cancer
Gang Ye
2TMR Integrative Medicine    2019, 3: e19007.   https://doi.org/10.12032/TMRIM201903007
Abstract HTML PDF (393KB)  

Highlights:

Banxiashengjiangdaizheshi decoction combined warm acupuncture have the function of invigorating stomach and removing turbid, warming yang and lowering reverse.

Abstract

Objective: To study the treatment of postoperative gastroparesis in patients with gastric cancer.Methods 18 patients with gastroparesis after gastric cancer surgery were divided into two groups, including 10 patients in the observation group and 8 patients in the control group. The observation group was treated with traditional Chinese medicine combined with warm acupuncture and moxibustion, and the control group was treated with western medicine.Results After 7 days treatment, there was significant difference in cure rate and total effective rate between the two groups (P < 0.01), and the treatment group was obviously superior to the control group.Conclusion Traditional Chinese medicine combined with warm acupuncture is effective in the treatment of postoperative gastroparesis of gastric cancer.

Shenqi Fuzheng injection combined with GP chemotherapy in the treatment of advanced non-small cell lung cancer: a meta-analysis
Qiang Zhang, Yi-Huan Fan, Teng Zhang, Xiao-Lan Qin, Ji-Fang Song
TMR Integrative Medicine    2017, 1 (2): 68-78.   https://doi.org/10.12032/TMRIM201701119
Abstract PDF (547KB)  
Objective: To evaluate the clinical efficacy of Shenqi Fuzheng injection combined with gemcitabine plus cisplatin (GP) in the treatment of advanced non-small cell lung cancer (NSCLC). Methods: we performed a systematic search in the electronic databases such as Cochrane Library, Pubmed, Embase, Chinese Journal Full-text Database, Chinese Biomedical Literature Database, Chinese Science and Technology Periodical Full-text Database and Wanfang Database up to 30 January 2017. Randomized controlled trials (RCT) of Shenqi Fuzheng Injection combined with GP chemotherapy in the treatment of advanced NSCLC were searched, and all the RCTs were conducted on methodological quality assessment. Data extraction and data analysis were according to standards of Cochrane systematic review. Results: Eight trials were included including a total of 701 patients. Meta-analysis results: Shenqi Fuzheng injection combined with GP chemotherapy could significantly improve the functional status of patients with NSCLC (OR = 3.44, 95% CI [2.26, 5.25], P < 0.0001) and clinical treatment efficacy (OR = (OR = 0.31, 95%CI [0.20, 0.47], P < 0.0001. The rate of leukopenia (OR = .31, 95%CI [0.20,0.47], P < 0.0001), thrombocytopenia (OR = 0.58, 95%CI [0.37, 0.91], P = 0.020), hemoglobin decline ((OR = 0.31, 95%CI [0.16, 0.59], P = 0.0004) and incidence of gastrointestinal reactions (OR = 0.58,P < 0.05) could be reduced. Conclusion: Shenqi Fuzheng injection combined with GP chemotherapy in the treatment of advanced NSCLC obtained significantly clinical efficacy. The quality of the literature incorporated is low, the conclusion requires high-quality research to further prove.
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