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6TMR Modern Herbal Medicine  2019, Vol. 2 Issue (3): 151-157    DOI: 10.12032/TMRmhm2017A50
Orginal Article     
Mitochondrial membrane stabilization by Angelica sinensis polysaccharide in murine aplastic anemia
Ping Zhong1, Xing Cui2()
1Department of rehabilitation medicine, The 960th hospital of the people's Liberation Army, Jinan, Shandong, China.
2Department of Hematology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong, China.
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Abstract

In order to investigate the mechanism of mitochondrial membrane stabilization by Angelica sinensis polysaccharide (ASP) in murine aplastic anemia (AA).ICR mice were randomly divided into control, AA and ASP-treated groups. The AA group mice were treated with 60Coγand intraperitoneal injections of cyclophosphamide and chloramphenicol. The control animals were treated with lead shielding irradiation and saline injection. The treated AA mice were fed with ASP for 2 wk. Mitochondrial ultrastructure of the bone marrow was observed by transmission electron microscopy, and the transmembrane potential of bone marrow-nucleated cells (BMNC)was examined by fluorescence spectrophotometry. The Cox and MDH contents of the medium were also studied in the three groups.The mitochondrial number and transmembrane potential of BMNC in the bone marrow decreased in the AA group as compared to the control group, but improved in the ASP-treated group as compared to the AA group. Complete mitochondrial cleavage in the ASP-treated group was significantly delayed (P < 0.05) as compared to the AA group. We conclude that ASP might improve mitochondrial membrane stabilization, and suppress the downregulation of transmembrane potential and apoptosis of BMNC in AA.

Highlights

Acquired deletions of mtDNA and abnormal mitochondrial function are crucial reasons in some blood disease include aplastic anemia. Angelica sinensis helps in tonifying the blood and promoting its circulation via anti-oxidative and neuroprotective effects. In this paper, we demonstrated that Angelica sinensis polysaccharide can improve improve the mitochondrial ultrastructure, and suppress the downregulation of transmembrane potential and apoptosis of myeloid element to cure bone marrow failure.



Key wordsaplastic anemia      Angelica sinensis polysaccharide      mitochondria      membrane potential      ICR mice     
Published: 25 July 2019
Fund:  This study was supported by the National Natural Science Foundation of China (No. 81202839), the National Natural Science Foundation of China (No. 81774080), the “Taishan Scholar” Project Special Fund, the Study Abroad Funding by the Shandong health science and technology association and the Affiliated Hospital of Shandong University of Traditional Chinese Medicine.
About author: # Both authors contributed equally to this work.
Cite this article:

Ping Zhong, Xing Cui. Mitochondrial membrane stabilization by Angelica sinensis polysaccharide in murine aplastic anemia. 6TMR Modern Herbal Medicine, 2019, 2(3): 151-157. doi: 10.12032/TMRmhm2017A50

URL:

https://www.tmrjournals.com/mhm/EN/10.12032/TMRmhm2017A50     OR     https://www.tmrjournals.com/mhm/EN/Y2019/V2/I3/151

Figure 1. Effects of ASP on the mitochondria of hematopoietic cells from AA mice.
After treatment with ASP or physiological saline for 2 wk, representative transmission electron micrographs (×25000) of mitochondrial structures from hematopoietic cells are shown (B and C). Panel A is the control group. Panel C shows more mitochondria in the ASP-treated group as compared to the AA group (Panel B).
Group After the establishment
of AA models (d)
n WBC ×109 Hb (g/l) PLT ×109 BMNC
(×106/thigh bone)
Control group 24 4.93±0.42 154.46±13.36 1084.00±60.31 3.67±0.45
AA group 1
14
24
20
2.20±0.45*
1.55±0.31*
135.26±11.65*
79.35±10.99*
889.90±55.36*
625.25±84.14*
0.31±0.42*
0.58±0.26*
ASP-treated AA group 1
14
24
21
2.34±0.27*
3.03±0.29*△
138.77±15.31*
101.65±15.74
876.56±28.35*
805.35±61.37*△
0.43±0.32*
1.27±0.21*△
Table 1 Peripheral blood cell counts (x±SD) in the three groups of mice
Control group AA group ASP-treated AA group
Mitochondrial number 9.32±1.15 2.91±0.57* 7.66±0.93 **△
Mitochondrial membrane potential 31.7±2.59 19.6±3.03* 27.1±2.98 **△
Table 2 Effect of ASP on the mitochondrial number and mitochondrial membrane potential(MMP)of bone marrow in mice(x±SD)
Figure 2. Effects of ASP on the mitochondrial membrane potential (MMP) in bone marrow cells of aplastic anemia (AA) mice. Bone marrow cells from AA mice and ASP-treated AA mice were stained with rhodamine 123.
Figure 3. The concentration time curves of COX and MDH
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