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6TMR Modern Herbal Medicine  2020, Vol. 3 Issue (1): 21-29    DOI: 10.12032/TMRmhm202003060
Article     
Protective effects of Pulsatilla chinensis Regel against isoproterenol-induced heart failure in mice

Huimin Zhang1, Congping Su1, Wei Wang1*, Sen Li2*, Shuzhen Guo1*

1 School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China;

2 School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China.

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Objective: To study the cardioprotective effect of Baitouwen (Pulsatilla chinensis Regel, PR) in isoproterenol (ISO) induced heart failure in mice, and investigated the molecular targets using network pharmacological prediction and experimental validation. Methods: PR were orally administered to ISO induced HF mice for two weeks. The cardiac function was analyzed by echocardiography. Hematoxylin-eosinstaining (HE) was used to evaluate the pathological changes. The PR targets for HF were predicted by bioinformatics analysis tool (BATMAN-TCM). The expression of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and HF related genes Phosphodiesterase (PDEs), were detected by real-time fluorescence quantitative PCR or Immunohistochemical staining in heart tissues. Results: Cardiac hypertrophy was observed after ISO treatment, and hypertrophic cardiomyocytes were arranged in disorder. Moreover, the expression of ANP and BNP gene in myocardium were upregulated (P < 0.05). Treatment of HF mice with PR aqueous extract improved cardiac function characterized by enhanced ejection fraction (EF) and fraction shortening (FS). Furthermore, PR extract also downregulated the expression of ANP and BNP in the heart tissues. Arrangement of cardiomyocytes was also improved. Furthermore, administration of PR group decreased target genes PDE5 and PDE1C expression in the hearts. In addition, PR treatment also improved myocardial fiber distribution and decreased myocardial thickness in the hearts of HF mice. Conclusion: PR treatment may suppress cardiac hypertrophy and improve myocardial function in HF mice via inhibiting the predicted targets PDE5 expression. These results suggest the potential of using PR in preventing the development of HF.


Key wordsPulsatilla chinensis (Bunge) Regel       Heart failure      Network pharmacology       PDE1       PDE5     
Received: 13 January 2020      Published: 01 February 2020
Fund:  

This study was supported by the Fundamental Research Funds for the Central Universities (NO. 2019-JYB-TD-002).

Corresponding Authors: Shuzhen Guo, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, No.11 Beisanhuan East Road, Chaoyang Disctrict, Beijing100029, China; Wei Wang, School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, No.11 Beisanhuan East Road, Chaoyang Disctrict, Beijing 100029, China; Sen Li, School of Life Sciences, Beijing University of Chinese Medicine, No.11 Beisanhuan East Road, Chaoyang Disctrict, Beijing 100029, China.   
E-mail: guoshz@bucm.edu.cn; Wangwei26960@126.com; senli@bucm.edu.cn
Cite this article:

Huimin Zhang, Congping Su, Wei Wang, Sen Li, Shuzhen Guo. Protective effects of Pulsatilla chinensis Regel against isoproterenol-induced heart failure in mice. 6TMR Modern Herbal Medicine, 2020, 3(1): 21-29. doi: 10.12032/TMRmhm202003060

URL:

https://www.tmrjournals.com/mhm/EN/10.12032/TMRmhm202003060     OR     https://www.tmrjournals.com/mhm/EN/Y2020/V3/I1/21

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