Dendrobium huoshanense improves doxorubicin-induced heart failure in C57BL/6 mice
TMR Modern Herbal Medicine. 2020, 3 (2): 55-65.
Objective: To investigate the therapeutic effect of Shihu (Dendrobium huoshanense, DH) on doxorubicin (DOX)-induced heart failure in mice and the involved mechanisms. Methods: Male C57BL/6 mice were randomly divided into 3 groups: Control (Ctrl) group, DOX group and DH group. Chronic heart failure was induced by intraperitoneal injection of doxorubicin solution. Mice in DH group were fed normal chow containing DH powder for 4 weeks. After 4-week treatment, electrocardiograms were measured. At the end of experiment, serum and heart sample were collected for determination of indicators for heart failure indicators. The heart tissues were conducted HE, Masson , Sirius red staining and TUNEL staining to determine cardiac tissue morphology, fibrosis, collagen content and apoptosis, respectively. mRNA and protein expression were determined by qRT-PCR and Western blot, respectively. Results: DH reduced the DOX-induced serum biomarkers (creatine kinase, aspartate aminotransferase and lactate dehydrogenase) of heart damage and reduced heart fibrosis. Mechanically, DH inhibited myocardial apoptosis, decreased interleukin 6 and tumor necrosis factor α levels, but increased superoxide dismutase 2 expression. Conclusion: DH alleviates DOX-induced chronic heart failure by inhibiting inflammatory pathway and enhancing anti-oxidative enzymes. Our study provides the potential of DH for heart failure treatment.
Effects of theophylline/chitosan/β-cyclodextrin microspheres for oral drug delivery on an asthmatic rat model
TMR Modern Herbal Medicine. 2020, 3 (2): 66-76.
Objective: This study aimed to investigate the effects of TH/CTS/β-CD microspheres by oral administration on ovalbumin allergic asthma of a rat model. Methods: Spray drying method was applied for preparing the TH/CTS/β-CD microspheres, which were used in treatment of asthmatic rats. At a predetermined time, the levels of eosinophil (Eos), protein, lactate dehydrogenase (LDH), aspartate transaminase (AST), glutamic pyruvic transaminase (GPT), and creatinine (Cr) were ascertained by automatic hematology and biochemical analyzers. Lung tissue histology was performed by hematoxylin-eosin staining. Results: No significant differences were found about the contents of Eos in the blood, and the contents of Eos, protein and LDH in the bronchoalveolar lavage fluid between the microsphere-treated and dexamethasone-treated groups, but the levels were lower in both treated groups than in the model group. In the microsphere-treated group, the levels of Cr, AST, and GPT in the serum of rats showed no significant differences compared with the normal group. Based on lung histopathological findings, the microspheres attenuated inflammatory cell infiltration and mucus hypersecretion compared with the model group. Conclusion: This study demonstrated that TH/CTS/β-CD microspheres exerted an anti-inflammatory effect and could serve as a novel promising drug delivery system for asthma treatment.
The functional components and mechanism of Linderae Radix in treating diabetic nephropathy based on the network pharmacology
TMR Modern Herbal Medicine. 2020, 3 (2): 77-85.
Objective: To analyze the components and mechanisms of Wuyao (Linderae Radix) in treating diabetic nephropathy (DN) based on network pharmacology. Methods: Multiple online databases were used to search and screen out the active ingredients from Linderae Radix, the related targets of active components of Linderae Radix and the genes related to DN. Search the corresponding genes name of target through UniProt database. Cytoscape 3.2.1 was used to construct the corresponding target gene network of Linderae Radix compounds.Venn diagram was used to screen the intersection genes of the active components corresponding to the target and disease-related genes, and the intersection genes were constructed into the protein interaction relationship network. Finally, DAVID database was used to do GO function enrichment analysis and KEGG signaling pathway enrichment analysis for the intersection genes, and the results of GO and KEGG were visualized. Results: 1. A total of 7 potential active ingredients and 100 target proteins were screened. 2. There are a total of 34 intersection genes between the potential active ingredient target in Linderae Radix in DN. 3. The top 10 of the interaction correlation between intersection proteins include:AR and NCOA2, NCOA2 and NR3C1, NCOA2 and PPARG, etc. 4. There were 16 entries of molecular function, 9 entries of cell component, 47 entries of biological process and 18 entries of signaling pathway (P <0.05). Conclusion: DN was treated by Linderae Radix from multi-component, multi-target and multi-link synergies.
Research progress on chemical composition, pharmacological effects of Forsythia suspensa (Thunb.) Vahl and predictive analysis on Q-marker
TMR Modern Herbal Medicine. 2020, 3 (2): 86-112.
Forsythia suspensa (Thunb.) Vahl, Abbreviated as “ F. suspensa ” below) is traditional Chinese medicine in China, widely distributed all over the country and also highly rich in resources. The Northwest Territories is a genuine producing area. There are many chemical components in F. suspensa, including lignans, phenylethanolic glycosides, flavonoids, C6-C2 natural alcohols and their glycosides, phenolic acids, terpenes and volatile oils. Among them, lignans and phenylethanolic glycosides are the main active components. Based on the review of chemical components or constituents and main pharmacological activities, according to the definition of quality marker, the components of quality marker of F. suspensa were predicted and analyzed from the aspects of plant genetics and chemical composition, traditional efficacy, traditional medicine, new efficacy, blood entering composition, measurable composition, effective composition in different compatibility, and the influence of storage conditions of extract. Further research on its medicinal active ingredients will provide scientific basis for the evaluation of F. suspensa quality.