The Protective Effect of Jiujiuguiyi, a Medicine and Food Homologous Formula, on Acute Alcohol Poisoning Mice
TMR Modern Herbal Medicine. 2019, 2 (3): 121-130.
Background: Nowadays, acute alcoholic intoxication has become the third public problem in China, and the anti-inebriation products mainly aimed at increasing the activity of enzyme involved in the alcohol metabolism, which is a single mechanism that can accelerate alcohol metabolism. Thus, a new formula, Jiujiuguiyi (JJGY) which could protect liver, relieve the abnormal excitability of the center and improve muscle retardation at the same time is designed by us. Methods: The model of acute alcoholic intoxication was established by intragastric administration with 0.12 ml/10g 50% alcohol in mice. JJGY was orally administrated (gavage) once a day for 20 consecutive days before the establishment of acute alcoholic model. Mice were randomly divided into 8 groups with 8 each: blank control group (CON), model group (M), Haiwangjinzun positive control group (HWJZ), experimental groups (AL, AH, BL, BH, AB). Giant, crawling time on the rota-rod, the activities of aspartate amino trans- ferase (AST), alanine amino transferase (ALT) and superoxide dismutase (SOD) in both liver and serum, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), glutathione peroxidase (GSH-PX) in liver as well as the HE staining of liver slices, the formation of malondialdehyde (MDA) in serum were determined after acute alcoholic intoxication. Results: Compared with model group, JJGY significantly decreased the AST and ALT activity in liver and serum and MDA activity in serum. Meanwhile, it enhanced the ADH and ALDH level in liver as well as the hepatic and serous SOD activity, indicating more efficient metabolism of alcohol and less hepatic injury. HE staining results also proved that JJGY could reduce alcoholic liver cell injury, and the effect was more obvious in the group medicated before alcohol administration. Moreover, JJGY significantly prolonged the crawling time on the rota-rod and improved the gait of mice and the effect was proved to be better than the widely used health product Haiwangjinzun. Conclusions: This study suggests that JJGY is able to protect liver, relieve the abnormal excitability of the center and improve muscle retardation after acute alcoholic intoxication. Its liver protection effect is likely related to its modulation on the alcohol metabolizing and antioxidant enzymes.
In the present study, a new compound Chinese herbal medicine formula,
Jiujiuguiyi, was designed by using the medicine and food homology theory. The formula aims at protecting liver, relieving the abnormal excitability of the center and improving muscle retardation at the same time. Acute alcoholic intoxication model in mice was built, then the ethology test and biochemical test were conducted to exam the efficacy of the formula in different preparations. The results suggest that JJGY can protect the acute alcoholic intoxication mice through multiple mechanisms, providing a new way to develop antialcoholismic drug homologous food.
Effect of Tanshinone IIA on LPS-induced inflammatory response in a ROS-NLRP3 inflammasome dependent manner in RAW264.7 cells
TMR Modern Herbal Medicine. 2019, 2 (3): 131-139.
Emerging evidence has demonstrated that Tanshinone IIA (Tan IIA) prevents cardiomyocytes injury, cardiac fibroblasts and atherosclerosis. However, the molecular mechanism underlying the effects of Tan IIA is still unclear. To investigate the role of Tan IIA in inflammatory response in a ROS-NLRP3 inflammasome dependent manner, RAW264.7 cells stimulated with LPS were recruited to produce a cell model of inflammatory response. Our results indicated that the production of NO was significantly increased after stimulated by LPS, and Tan IIA treated significantly decreased the level of NO. The mRNA expression of NLRP3, IL-1β and TNF-α was significantly inhibited by Tan IIA compared with LPS treated cells. The protein expression of NLRP3, IKBα, pp65/p65 and pp38/p38 was significantly decreased by Tan IIA, compared with LPS or LPS+ATP stimulated groups. Meanwhile, Tan IIA significantly inhibited the level of ROS induced by LPS+ATP. And NAC, a ROS inhibitor, could also inhibit the protein expression of NLRP3. Based on these findings, it could be speculated that the mechanism underlying the effect of Tan IIA may involve the regulation of ROS-NF-κB/ P38-NLRP3 pathway. This study further characterized the molecular mechanism of Tan IIA, and provided new thoughts to its clinical therapy.
Tan IIA could inhibit the inflammatory response and NLRP3 expression stimulated by LPS or LPS+ATP. Acetylcysteine (N-acetyl-l-cysteine, NAC), a ROS inhibitor, could inhibit LPS+ATP-induced increase in NLRP3 level. The mechanism underlying the effects of Tan IIA may involve the regulation of ROS-NF-κB/ P38-NLRP3 pathway. This study further characterized the molecular mechanism of Tan IIA, and provided new thoughts to its clinical therapy.
Analysis of drug use law and mechanism of prostate cancer based on data mining and network pharmacology
TMR Modern Herbal Medicine. 2019, 2 (3): 140-150.
Objective: Excavate the medication rule of traditional Chinese medicine in the treatment of prostate cancer, and predicting the biomolecular level mechanism of high-frequency drug compatibility. Methods: Relevant documents in CNKI, Wanfang Medical Network and VIP Chinese Biomedical Periodical Database Pubmed, EMbase were collected and collated systematically. Frequency statistics, association rule analysis and new party mining were carried out using TCMISSV2.5. BATMAN-TCM was used to analyze the interaction relationship and related pathways between high-frequency drug targets. Results: Huangqi ( Astragalus membranaceus) was the single drug most used of the 102prescriptions included in the standard. There are 6 pairs of combinations with high confidence in association rule analysis. System entropy cluster analysis resulted in 20 core drug combinations and 9 new prescriptions. Through KEGG pathway analysis of Huangqi , Fuling ( Poria cocos) , Gancao ( Glycyrrhiza uralensis) and Dihuang ( Rehmannia glutinosa), it was found that the number of potential targets of the neural active ligand receptor rented pathway and purine metabolism pathway was the largest. Conclusions: Prostate cancer is mainly treated with deficiency-tonifying drugs, which are combined with drugs for promoting blood circulation, removing blood stasis, clearing heat, promoting diuresis, detoxifying and resolving hard mass. The mechanism of action of high-frequency traditional Chinese medicine may be realized by interfering with the neuroactive ligand receptor interaction pathway and purine metabolism pathway.
This article found that deficiency-tonifying drugs, which are combined with drugs for promoting blood circulation, removing blood stasis, clearing heat, promoting diuresis, detoxifying and resolving hard mass are commonly used to treat prostate cancer. Core herbs may play their role by interfering with the neuroactive ligand-receptor interaction pathway and purine metabolic pathway.
Mitochondrial membrane stabilization by Angelica sinensis polysaccharide in murine aplastic anemia
TMR Modern Herbal Medicine. 2019, 2 (3): 151-157.
In order to investigate the mechanism of mitochondrial membrane stabilization by Angelica sinensis polysaccharide (ASP) in murine aplastic anemia (AA).ICR mice were randomly divided into control, AA and ASP-treated groups. The AA group mice were treated with 60Coγand intraperitoneal injections of cyclophosphamide and chloramphenicol. The control animals were treated with lead shielding irradiation and saline injection. The treated AA mice were fed with ASP for 2 wk. Mitochondrial ultrastructure of the bone marrow was observed by transmission electron microscopy, and the transmembrane potential of bone marrow-nucleated cells （BMNC）was examined by fluorescence spectrophotometry. The Cox and MDH contents of the medium were also studied in the three groups.The mitochondrial number and transmembrane potential of BMNC in the bone marrow decreased in the AA group as compared to the control group, but improved in the ASP-treated group as compared to the AA group. Complete mitochondrial cleavage in the ASP-treated group was significantly delayed (
P < 0.05) as compared to the AA group. We conclude that ASP might improve mitochondrial membrane stabilization, and suppress the downregulation of transmembrane potential and apoptosis of BMNC in AA.
Acquired deletions of mtDNA and abnormal mitochondrial function are crucial reasons in some blood disease include aplastic anemia. Angelica sinensis helps in tonifying the blood and promoting its circulation via anti-oxidative and neuroprotective effects. In this paper, we demonstrated that Angelica sinensis polysaccharide can improve improve the mitochondrial ultrastructure, and suppress the downregulation of transmembrane potential and apoptosis of myeloid element to cure bone marrow failure.
Erahertz spectral analysis of Xiling Zhimu with different geological conditions and plant age
TMR Modern Herbal Medicine. 2019, 2 (3): 158-166.
Objective: In order to identify the quality of different geological conditions and plant ages of Xiling Zhimu. Methods: This article uses the terahertz spectroscopy to detect and analyze different geological conditions and ages of Zhimu ( Anemarrhenae Rhizoma) from the same origin. Results: The terahertz time-domain spectroscopy of different geological conditions and ages has a decrease relative to the reference amplitude and has a delay. The refractive index has obvious differences, and the refractive index can be used as a way to identify the tablets. The absorption coefficients of different ages Zhimu samples has significant differences in the range of 1.0-2.0 THz. The absorption coefficients of different geological conditions Zhimu samples has significant differences in the range of 1.1-2.0 THz. Conclusions: The differences caused by different production methods should be considered in the identification of genuine regional drug. When the Zhimu is grown due to different geological conditions and plant ages, its efficacy will also change. we can detect this change by terahertz spectroscopy.
The terahertzian time-domain spectrum of Zhimu (
Anemarrhenae Rhizoma) at different plant ages and different geological environments decreased relative to the reference amplitude and showed a delay. The refractive index has obvious difference, which can be used as a way to identify the film. The absorption coefficients of Zhimu at different plant ages were significantly different in the range of 1.0-2.0THz, and the absorption coefficients of Zhimu in different geological environments were different in the range of 1.1-2.0THz. Therefore, the differences caused by different preparation methods should be considered in the identification of authentic medicinal materials. When Zhimu grows at different plant ages and in different geological environments, its pharmacodynamics will change, and we can detect this change by terahertz spectroscopy, which can provide a basis for the identification of Xiling Zhimu.
Application Progress of Porous Materials in Modern Pharmaceutical
TMR Modern Herbal Medicine. 2019, 2 (3): 167-172.
Porous materials have regular three-dimensional pore structure, which has unique advantages in the field of modern pharmaceutical. At present, porous materials commonly used in the pharmaceutical field are mainly molecular sieves, macroporous adsorbent resins, activated carbon, etc. In this paper, the application status of these porous materials in the pharmaceutical field is reviewed, and the future development is prospected.
In order to solve the problems of difficult separation and purification of drugs and poor sustained release performance, researchers began to pay attention to porous materials with regular micro-porous structure. Because of their excellent performance, safety, non-toxicity, low cost and easy production, the common porous materials such as molecular sieves, macroporous adsorption resins and activated carbon are not only widely applied to industry, but also widely studied in the field of medicine. This paper summarizes the progress of the above porous materials in the field of medicine. It is found that these porous materials can be used to extract natural drugs and remove heavy metal impurities in drugs when promoting drug separation and purification, and they can reduce toxicity of raw materials, control drug release time and improve drug targeting when improving drug sustained release performance.