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Analysis of drug use law and mechanism of prostate cancer based on data mining and network pharmacology
Yao Yang, Ying Chen, Zhen-ning Yang, Guo-wei Zhang
6TMR Modern Herbal Medicine    2019, 2 (3): 140-150.   https://doi.org/10.12032/TMRmhm2017A52
Abstract ( 132 HTML PDF (1065KB) ( 90 )  

Abstract

Objective: Excavate the medication rule of traditional Chinese medicine in the treatment of prostate cancer, and predicting the biomolecular level mechanism of high-frequency drug compatibility. Methods: Relevant documents in CNKI, Wanfang Medical Network and VIP Chinese Biomedical Periodical Database Pubmed, EMbase were collected and collated systematically. Frequency statistics, association rule analysis and new party mining were carried out using TCMISSV2.5. BATMAN-TCM was used to analyze the interaction relationship and related pathways between high-frequency drug targets. Results: Huangqi (Astragalus membranaceus) was the single drug most used of the 102prescriptions included in the standard. There are 6 pairs of combinations with high confidence in association rule analysis. System entropy cluster analysis resulted in 20 core drug combinations and 9 new prescriptions. Through KEGG pathway analysis of Huangqi, Fuling (Poria cocos), Gancao (Glycyrrhiza uralensis) and Dihuang (Rehmannia glutinosa), it was found that the number of potential targets of the neural active ligand receptor rented pathway and purine metabolism pathway was the largest. Conclusions: Prostate cancer is mainly treated with deficiency-tonifying drugs, which are combined with drugs for promoting blood circulation, removing blood stasis, clearing heat, promoting diuresis, detoxifying and resolving hard mass. The mechanism of action of high-frequency traditional Chinese medicine may be realized by interfering with the neuroactive ligand receptor interaction pathway and purine metabolism pathway.

Highlights

This article found that deficiency-tonifying drugs, which are combined with drugs for promoting blood circulation, removing blood stasis, clearing heat, promoting diuresis, detoxifying and resolving hard mass are commonly used to treat prostate cancer. Core herbs may play their role by interfering with the neuroactive ligand-receptor interaction pathway and purine metabolic pathway.

Bio-characteristic profiling related to clinic: A new technology platform for quality evaluation of Chinese materia medic
Li-Na Wen, Ai-Ting Wang, Na Guo, Yumei Han, Can Yan, Dan Yan
6TMR Modern Herbal Medicine    2019, 2 (4): 215-224.   https://doi.org/10.12032/TMRmhm2017B54
Abstract ( 149 PDF (859KB) ( 94 )  
Quality evaluation is a bottleneck restricting the modernization and internationalization of Chinese materia medica (CMM). Due to characteristics of multi-component, multi-efficacy, multi-target, the existing quality evaluation system still cannot fully meet quality control needs of CMM. Hence, the author put forward “Bio-characteristic profiling related to clinic, BPRC” academic concept, meanwhile, fully take the advantage of analysis method with clinical monitoring superiority or profiling characteristics, build up "BPRC" new technology platform, in order to realize “real-time, dynamic and full-range monitoring” new technologies of CMM quality evaluation system and promote the further development of CMM industry.
Similarity measurement of Chinese medicine ingredients for cold-hot nature identification
Guo-Hui Wei, Xian-Jun Fu, Zhen-Guo Wang
6TMR Modern Herbal Medicine    2019, 2 (4): 183-191.   https://doi.org/10.12032/TMRmhm2017A58
Abstract ( 92 PDF (478KB) ( 155 )  
Objective: Nature theory of Chinese medicine (CM) is the core basic theory of Traditional Chinese Medicine (TCM), in which cold-hot nature is the focus of research. Studies have found that CM ingredients are the material basis for the production of medicine natures. Therefore, it is speculated that CMs with similar composition of substances should have similar medicinal nature. Modern work studies cold-hot medicine of CMs with chemical fingerprinting technology because the chemical fingerprint data of CM can reflect the whole composition of CM ingredients. Methods: To verify the hypothesis above, in this work, we study quantifying the similarity of CM ingredients to fingerprint similarity, and explore the relationship between the composition of CMs and cold-hot nature. Firstly, we utilize ultraviolet (UV) spectrum technology to analyze 61 CMs, which have clear cold-hot nature (including 30 ‘cold’ CMs and 31 ‘hot’ CMs). Secondly, with the constructed fingerprint database of CMs, a distance metric learning algorithm is studied to metric the similarity of UV fingerprints. Finally, a retrieval scheme is proposed to build a predictive identification model to identify cold-hot nature of CMs. Results: By means of numerous experiment analyses, ultraviolet spectrum data of petroleum ether solvent can better represent CMs to distinguish between cold and hot natures. Comparing with existing classical models, the proposed identification scheme has better predictive performance. Conclusion: The experimental results prove our inference that CMs with similar composition of substances should have similar medicinal nature. The proposed prediction model is proved to be effective and feasible.
Terahertz spectral analysis of Yunpian Lurong
Li-Na Zhang, Yu-Hui Ma, Kai-Xuan Hou, Ze-Jia Zhang, Yan-Ming Wu, Guo-Wei Zhang
6TMR Modern Herbal Medicine    2019, 2 (2): 83-90.   https://doi.org/10.12032/TMRmhm2017A47
Abstract ( 183 HTML PDF (510KB) ( 82 )  

Objective: Provide a rapid and simple method to identify Yunpian Lurong. Method: Terahertz spectroscopy was used to detect Yunpian Lurong. Result: Compared with the reference wave, the spectral amplitude of Yunpian Lurong decreases significantly. The frequency - domain spectra of Yunpian Lurong are different from conventional Lurong. Compared with the spectrum of free space, both of them have obvious absorption to THz wave, but the intensity and location of absorption are different, which provides a basis for the identification of Yunpian Lurong. The refractive index of the two samples is obviously different, which may be related to the different internal structure and composition content of the samples. The absorption coefficient of the two samples are different as well, when the frequency ranges from 0 to 2.4 THz, the absorption coefficients of the two kinds of Lurong samples have obvious difference, which makes it easy to distinguish the Yunpian Lurong. Conclusion: This study proves that terahertz spectroscopy can be used as an efficient and convenient method to identify Yunpian Lurong.

Highlights
Yunpian Lurong is one of the "four wonders of Qizhou". It is of great significance to develop the method for testing its commercial specifications. At present, many scholars have applied the fingerprint technology of traditional Chinese medicine to identify the LuRong, which is complex, time-consuming and costly for sample pretreatment is needed before testing. Therefore, it is necessary to establish a nondestructive and rapid detection and analysis method to identify Yunpin Lurong. In the experiment, under the constant and dry room temperature, we used the terahertz spectroscopy to detect Yunpin Lurong, analyzed and compared the differentiation of erahertz time domain spectroscopy, frequency domain spectroscopy, refractive index and absorption spectroscopy between Yunpin Lurong and the conventional Lurong. The results show that Yunpin Lurong can be quickly identified by terahertz spectroscopy without destroying its surface and internal structure or complicated pretreatment. As a new detection technology, it has the advantages of simple operation, short time-consuming, low cost and pollution-free and a positive meaning for the rapid detection of Yunpian Lurong, which is a nondestructive testing method for quality analysis or identification of Chinese medicinal herb with a promising prospect.

Mitochondrial membrane stabilization by Angelica sinensis polysaccharide in murine aplastic anemia
Ping Zhong, Xing Cui
6TMR Modern Herbal Medicine    2019, 2 (3): 151-157.   https://doi.org/10.12032/TMRmhm2017A50
Abstract ( 141 HTML PDF (647KB) ( 75 )  

Abstract

In order to investigate the mechanism of mitochondrial membrane stabilization by Angelica sinensis polysaccharide (ASP) in murine aplastic anemia (AA).ICR mice were randomly divided into control, AA and ASP-treated groups. The AA group mice were treated with 60Coγand intraperitoneal injections of cyclophosphamide and chloramphenicol. The control animals were treated with lead shielding irradiation and saline injection. The treated AA mice were fed with ASP for 2 wk. Mitochondrial ultrastructure of the bone marrow was observed by transmission electron microscopy, and the transmembrane potential of bone marrow-nucleated cells (BMNC)was examined by fluorescence spectrophotometry. The Cox and MDH contents of the medium were also studied in the three groups.The mitochondrial number and transmembrane potential of BMNC in the bone marrow decreased in the AA group as compared to the control group, but improved in the ASP-treated group as compared to the AA group. Complete mitochondrial cleavage in the ASP-treated group was significantly delayed (P < 0.05) as compared to the AA group. We conclude that ASP might improve mitochondrial membrane stabilization, and suppress the downregulation of transmembrane potential and apoptosis of BMNC in AA.

Highlights

Acquired deletions of mtDNA and abnormal mitochondrial function are crucial reasons in some blood disease include aplastic anemia. Angelica sinensis helps in tonifying the blood and promoting its circulation via anti-oxidative and neuroprotective effects. In this paper, we demonstrated that Angelica sinensis polysaccharide can improve improve the mitochondrial ultrastructure, and suppress the downregulation of transmembrane potential and apoptosis of myeloid element to cure bone marrow failure.

Toxic effects of BuGuZhi and feasibility analysis of ways to synergistic and attenuated
Cui Qian, Ma Yu-Hui, Jin Xiao-Ming, Liu Jing-Jing, Zhang Yan-Hong, Zhang Guo-wei
TMR Modern Herbal Medicine    2018, 1 (4): 241-248.   https://doi.org/10.12032/TMRmhm2017B30
Abstract ( 311 HTML PDF (347KB) ( 211 )  

BuGuZhi (Psoraleae Fructus) is the dry mature peel of the leguminous plant. After ripening in autumn, the fruit is collected and dried to get its fruit, which has the effect of warming kidney and helping Yang, the effect of absorbing asthma, warming the kidney and relieving diarrhea, and treating vitiligo and alopecia. Because the clinical curative effect is exact, so it has been widely used in the treatment of clinical diseases. However, the recent reports and clinical cases reported that BuGuZhi has some toxic effects. According to domestic and foreign references, toxic effects are mainly divided into hepatotoxicity, nephrotoxicity, phototoxicity, reproductive toxicity, and rare cases of retinal vagueness reported by clinical reports. the toxicity of BuGuZhi is summarized. The article analyzed the way of synergism and reduction of BuGuZhi, which was based on data mainly coming from the compatibility of drugs, pattern identification, constitution identification and drug processing. All o-f analyses and summaries are in order to make the clinical medication safer and more scientific.

Highlights

BuGuZhi (Psoraleae Fructus),belonging to angiosperm, dicotyledoneae, rosidae , leguminosae, Psorslea L. Psoraleae Fructus, which was first recorded in “Kai Bao Ben Cao”, has been used widely in clinics for the functions of warming the kidney and helping the yang, calming the breath, warming the kidney, stopping the diarrhea. As the incidence of adverse reaction events accumulated in recent years, it has attracted the attention of domestic and overseas scholars. Thus, in order to provide a basis for doctors to use BuGuZhi safely, this article summarizes the literature on the toxicity of BuGuZhi, and analyzes the ways of enhancing efficacy and reducing toxicity.

Evidence-based optimization of integrated traditional Chinese and Western medicine therapies for prevention and treatment of coronary heart disease: design and implementation
Jiang Yin, Zhang Xiao-Yu, Tian Gui-Hua, Zhang Li-Jing, Zhang Ming-Xue, Wan Hai-Tong, Ye Min, He Rong-Rong, Sun Xin, Shang Hong-Cai
TMR Modern Herbal Medicine    2018, 1 (3): 124-126.   https://doi.org/10.12032/TMRmhm2017B28
Abstract ( 433 HTML PDF (254KB) ( 254 )  

As a chronic disease that seriously endangers public health, the number of coronary heart disease (CHD) patients in China has increased in recent years. There is great potentiality to integrate traditional Chinese and Western medicine therapies to prevent and treat CHD in clinical practice. However, most of the current integrated therapies still lack sufficient high-quality evidence, and the key links in how to apply are unclear. It is urgent to optimize them through evidence-based research to further improve the effectiveness. Therefore, we propose strategies to conduct evidence-based optimization of integrated traditional Chinese and Western medicine therapies in prevention and treatment of progressive cardiovascular diseases. These are integrated clinical trial design, attention to the key links of taking effect, combination of clinical and basic research. Based on the strategies, we started the national key research and development project "Evidence-based optimization research of TCM therapies in prevention and treatment of CHD (angina pectoris - myocardial infarction - heart failure)", which is expected to be a new paradigm in the field.

Effect of splitting combination of different components of Zhilong Huoxue Tongyu Capsule on vascular remodeling in hypertension
Liao Hui-ling, Ma Qian, Fu Ling
TMR Modern Herbal Medicine    2018, 1 (3): 164-170.   https://doi.org/10.12032/TMRmhm2017A24
Abstract ( 413 HTML PDF (605KB) ( 200 )  

Objective: To observe the effects of the components of Zhilong Huoxue Tongyu Capsule (ZLHXTY) on the expression of NF-κB-P65 and ICAM-1 in blood vessels of rats, and to explore the effect and mechanism of ZLHXTY and its ingredients on vascular remodeling in hypertension. Methods: A rat model of renal hypertension was established by narrowing the left renal artery in the operation group. The corresponding drugs were given once a day respectively. The Normal group, Sham-operation group and Model group were administered with the same volume of normal saline. After 4 weeks of gavage, the thoracic aorta of rat was taken, followed by fixation, embedding, sectioning and HE staining, and NF-κB -p65 was detected by immunohistochemistry. Results: 1. The effect of drugs on the expression of NF-κB in thoracic aorta of hypertensive rats (immunohistochemistry): A small amount of NF-κB protein was expressed in the Normal group and the Sham-operate group. The NF-κB expression in the Model group was significantly increased, and the electron microscope image showed that the brown-yellow granule was distributed in the vascular smooth muscle of the membrane. After treatment, the expression of NF-κB in the Captopril group and the Whole prescription group was significantly reduced compared with the Model group, while the protein expression in other groups was decreased compared with the Model group. The protein expression of captopril group was decreased compared with that of the Whole prescription group. Compared with Promoting blood circulation for removing blood stasis group, the expression of NF-κB in Benefiting qi group was significantly decreased and was similar to that in Warming meridian group. Conclusion: The vascular remodeling mechanism of ZLHXTY on renal hypertension rats may be related to its effects on lowering blood pressure, antioxidant stress and anti-inflammation. After the compatibility of formula produced a significant synergistic effect, and its compatibility after embodies multiple targets for treatment of advantages of traditional Chinese medicine.

Highlights

By observing the effect of each component of Zhilong Huoxue Tongyu Capsule (ZLHXTY) on the expression of blood vessel NF-κB -P65 and ICAM-1 in rats, to explore the effect of ZLHXTY and its ingredients on vascular remodeling in hypertension and its mechanism. The experimental results indicate that different components of ZLHXTY exert therapeutic effects on vascular remodeling in hypertensive rats from different aspects. Huangqi (Radix Astragali seu Hedysari) plays an important role in lowering blood pressure. The combination of Guizhi (Ramulus Cinnamomi) and Daxueteng (Caulis Sargentodoxae) has a significant effect on the expression of NF- induced B protein. Shuizhi (Hirudo) and compatibility of Dilong (Lumbricus) can lower blood pressure, the NF-κB protein expression. The synergistic effects of the combination of various components are significant, which reflects the therapeutic advantages of multiple targets of traditional Chinese medicine.

Prospects for the future of clinical efficacy evaluation of traditional Chinese medicine——Emphasis on original theory and clinical practice
Chen Zhao, Wei Mu, Hongcai Shang
6TMR Modern Herbal Medicine    2020, 3 (1): 1-5.   https://doi.org/10.12032/TMRmhm202003057
Abstract ( 102 PDF (280KB) ( 61 )  
Traditional Chinese medicine (TCM) clinical efficacy evaluation runs through the development of TCM and has been progressing so far. With the introduction of evidence-based medicine (EBM) in recent 20 years, TCM clinical efficacy evaluation has developed rapidly, accumulated research and experience, improved the quality and standardization of research, but also gradually found deep-seated deficiencies. In 2019, the China Center for Evidence-based Traditional Chinese Medicine (CCEBTCM) was established, and TCM was included in the 11th revision of the International Statistical Classification of Diseases (ICD-11). In above opportunities for TCM clinical efficacy evaluation, it is necessary to face up to the previous deficiencies, so that the TCM clinical efficacy evaluation can truly reflect the actual clinical practice characteristics, including TCM clinical problems, TCM thinking and TCM diagnosis & treatment mode. Based on the summary and analysis of past research, development concepts are proposed in this article. On the one hand, original concepts of TCM should be confidently paid more attention to the design and implementation of TCM clinical efficacy evaluation to be close to TCM clinical practice. On the other hand, it is necessary to consolidate the foundation of TCM clinical practice before evaluating efficacy. Evaluated object built on TCM ontology knowledge should be demonstrated based on clinical practice, and unknown evaluated object combined with new methods, technologies and concepts should be maturely integrated in clinical practice firstly.
Effects of Angelica Polysaccharide on telomere length in mice with benzene-induced aplastic anemia
Runjie Sun, Xing Cui, Qingsong Wang, Yanyu Zhang, Xiaoqi Sun
6TMR Modern Herbal Medicine    2020, 3 (1): 6-12.   https://doi.org/10.12032/TMRmhm202003059
Abstract ( 54 PDF (456KB) ( 38 )  
Objective: To investigate the changes in telomere length and the level of burst-forming units-erythrocyte (BFU-Es) and colony-forming unit-erythrocytes (CFU-Es) in mice with benzene-induced aplastic anemia (AA), and follow-up the therapeutic effects of Angelica Polysaccharide (AP). Methods: Male BALB/c mice (n = 120) were randomly divided into three groups (1, 2, 3): normal control (n = 24), AA control (n = 48), and treated AA (n = 48), respectively. Mice in Group 2 received benzene inhalation for 2.5 months and 1 ml distilled water p.o. per day for 2 weeks after the establishment of AA models. Similar procedure was applied to the mice in Group 3 and AP was given for 2 weeks after the establishment of AA models. Real-time polymerase chain reaction was used to survey relative telomere length measurement of the bone marrow cells. A BFU-Es and CFU-Es survey was done to follow-up the therapeutic effects of AP. Results: Compared with normal control, significant reductions of RBC, WBC, and platelet counts were found in peripheral blood of AA mice. After treatment with AP, counts of BFU-Es and CFU-Es were restored up to 66.8% and 77.25%, respectively, and length of telomere was restored up to 76.34%, of the normal levels. The telomere length in treated AA group was higher than the control AA group. Conclusion: The AP can protect the telomere length and differentiation of hemopoietic stem/progenitor cells, accelerate the recovery of BFU-Es and CFU-Es of AA mice, and then improve the bone marrow failure.

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