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Traditional Medicine Research  2017, Vol. 2 Issue (1): 27-32    DOI: 10.12032/TMR201705034
Basic Research     
Research on Xijiao Dihuang Decoction suppressing platelet apoptosis in immune-mediated aplastic anemia based on mitochondrial mediated pathway
Xia Le-Min, Cui Le-Le, Jiang Yi-Ling, Zheng Qin, Zhang Ai-Ping, Luo Mei-Hong*()
Department of Hematology, Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Baoshan Branch of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201999, China.
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Excessive platelet apoptosis is one of the pathogenic cause for immune-induced aplastic anemia. In the present study, we established an immune-induced aplastic anemia model and gave Xijiao Dihuang Decoction lavage daily. The results of this study indicated Xijiao Dihuang Decoction could increase platelet number and prevent its apoptosis through the mitochondrial pathway.


The aplastic anemia mice model was established in this study. BALB/c mice shall be treated with whole body irradiation with 60 Co-γradiation (5.5Gy, 1.1Gy/min × 5 min). then within 4 h, DBA/2 mice were injected lymphocyte suspension 1 × 106 cells /mouse through caudal vein. Grouping of testing animals: Normal control mice were healthy C57BL/6 mice without AA modeling. AA control group mice were exposed to radiation and cell transfusion and had no treatment with either CSA or XDD. CSA group mice received daily lavage with 0.027g/kg (0.1ml/10g) of CSA whereas XDD group mice received daily lavage with 19.5g/kg of XDD. The experimental result indicated CSA and XDD lavage mice had significantly higher platelet count and ΔΨm than AA mice (P < 0.05). Levels of Cyt C, PS and Ca2+ were significantly lower in CSA and XDD groups compare with AA group. More specifically, compared to CSA group, XDD group also had lower level of ΔΨm and higher level of Cyt C and Ca2+ (both P < 0.05). Both CSA and XDD treatment reduced Bak and Bax levels significantly compared to AA mice. However, XDD treatment still showed higher expressions than CSA (P < 0.05). CSA and XDD treatment increased the levels of Pro-apoptotic protein expressions ( caspase-8, caspase-3, caspase-9). XDD showed less potent effect than CSA in increasing these protein expressions (all P < 0.05). Therefore, we hypothesized XDD was possible to increase platelet number and prevent its apoptosis in immune-induced AA via the mitochondrial pathway.

Key wordsAplastic anemia      Xijiao Dihuang Decoction      Mitochondrial pathway      Apoptosis     
Published: 05 January 2017
Corresponding Authors: Luo Mei-Hong     E-mail:
About author: §These authors contributed equally to this work. Executive Editor: Cui-Hong ZhuEnglish Editor: Xiao-Hui Liu
Cite this article:

Xia Le-Min, Cui Le-Le, Jiang Yi-Ling, Zheng Qin, Zhang Ai-Ping, Luo Mei-Hong. Research on Xijiao Dihuang Decoction suppressing platelet apoptosis in immune-mediated aplastic anemia based on mitochondrial mediated pathway. Traditional Medicine Research, 2017, 2(1): 27-32. doi: 10.12032/TMR201705034

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Group PLT(×109 /L)
Normal control 614.20±53.90
AA control 352.40±53.56 a
CSA group 580.30±88.86 b
XDD group 547.50±70.84 b
Table 1 Platelet count number in four groups of mice (\(\overline{x}\) ± SD)
Group ΔΨm % Cyt C % PS % Ca2+ %
Normal control 0.84±0.07 0.19±0.01 0.04±0.01 65.13±3.79
AA control 0.20±0.02 a 0.89±0.04 a 0.73±0.11a 340.02±25.64 a
CSA group 0.60±0.07 ab 0.33±0.05 ab 0.55±0.08 ab 96.49±5.95 ab
XDD group 0.33±0.03 abc 0.51±0.08 abc 0.49±0.20 ab 252.45±6.25 abc
Table 2 The mitochondrial transmembrane potential and biochemics in four groups of mice (\(\overline{x}\)±SD)
Group Bak Area/b-actin Bax Area/b-actin Caspase-3 Area/b-actin Caspase-8 Area/b-actin Caspase-9 Area/b-actin
Normal control 0.21±0.02 0.14±0.01 0.63±0.03 0.63±0.03 0.64±0.03
AA control 0.49±0.04a 0.52±0.04a 0.24±0.03a 0.24±0.02a 0.23±0.03a
CSA group 0.23±0.02ab 0.20±0.03ab 0.48±0.02ab 0.47±0.04ab 0.47±0.04ab
XDD group 0.35±0.04abc 0.40±0.03abc 0.39±0.06abc 0.40±0.07abc 0.40±0.04abc
Table 3 Pro-apoptotic protein expressions in four groups of mice (\(\overline{x}\)±SD)
Figure 1 Pro-apoptotic protein expressions by western blotting.
Note: A: normal control; B: AA control; C: CSA group; D: XDD group.
Figure 2 XDD inhibits platelet apoptosis in immune-induced bone marrow failure through mitochondrial pathway.
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