Please wait a minute...
Traditional Medicine Research  2017, Vol. 2 Issue (3): 144-148    DOI: 10.12032/TMR201707051
Special Therapy of Traditional Medicine     
Effects of saponin from the seed of Litchi chinensis Sonn on TGF-β1, FN and SOCS-1 in renal tubular epithelial cells under high glucose
Nie Hai-Yang1, Chen Rui1,*(), Zhang Hong-Na2, Pan Zhi1
1Changchun University of Chinese Medicine, Changchun, China.
2Shunyi Hospital of Beijing Chinese Medicine Hospital, Beijing, China.
Download: HTML     PDF(541KB)
Export: BibTeX | EndNote (RIS)      

Highlights

Saponin from the seed of Litchi chinensis Sonn reduces the apoptosis of renal tubular epithelial cells and the secretion of TGF-β1 and FN.

Editor’s Summary

The seed of Litchi chinensis Sonn was first recorded as herb in Bencaogangmu in Ming Dynasty of China (1518 A.D. - 1593 A.D.). This study suggests that saponin from the seed of Litchi chinensis Sonn can be used to prevent the progress of glomerular sclerosis of diabetic nephropathy.

Abstract:

Objective: To investigate the effect of saponin from the seed of Litchi chinensis Sonn (SLS) on the growth and apoptosis of human kidney epithelial cells (HKC) cultured in high glucose. Methods: HKC were cultured in DMEM/F12 medium supplemented with 30 mmol/L glucose and treated with or without SLS. In the normal group, isometric DMEM/F12 medium with 5.5mmol/L glucose was added. The secretion of TGF-β1 and fibronectin (FN) were detected by ELISA. Cell apoptosis was detected by the method of Annexin V-FITC/PI double staining. Western blot was used to detect the level of suppressor of cytokine signaling-1 (SOCS-1). Results: The result of ELISA showed that the secretion of TGF-β1 and FN was decreased in SLS groups compared with those in 30 mmol/L glucose treated group (P < 0.05). There were more cells apoptosis in 30 mmol/L glucose treated group than that in the normal group (P < 0.01). Compared with the 30 mmol/L glucose treated group, the apoptosis of HKC were significantly decreased in SLS groups (P < 0.01). Western blot showed that the level of SOCS-1 in high glucose + SLS group was decreased (P < 0.01), compared with the high glucose group. Conclusion: SLS can reduce the secretion of TGF-β1 and FN in HKC by reducing the deposition of extracellular matrix. SLS also significantly reduced the apoptosis of HKC by inhibiting the level of SOCS-1. These results suggest the roles of SLS in preventing the progress of glomerular sclerosis.



Key wordsSaponin      Seed of Litchi chinensis Sonn      Glucose      Kidney epithelial cells      TGF-β1      Fibronectin      Apoptosis     
Published: 05 July 2017
Fund:  The study was supported by the National Natural Science Foundation of China (No. 81374025) and the Education Department of Jilin Province “13th Five-Year” science and technology research project.
Corresponding Authors: Chen Rui     E-mail: 794558014@qq.com
About author: Executive Editor: Cui-Hong Zhu English Editor: Lin Li, Li-Yun-Cui, Ya-Song Wang, Xiao-Hua Zhang, Xiao-Dong Wang, Hong-Xu Zhu, Zhen-Yan Liu, Yi-Cheng Shi
Cite this article:

Nie Hai-Yang, Chen Rui, Zhang Hong-Na, Pan Zhi. Effects of saponin from the seed of Litchi chinensis Sonn on TGF-β1, FN and SOCS-1 in renal tubular epithelial cells under high glucose. Traditional Medicine Research, 2017, 2(3): 144-148.

URL:

https://www.tmrjournals.com/tmr/10.12032/TMR201707051     OR     https://www.tmrjournals.com/tmr/EN/Y2017/V2/I3/144

Figure 1 Effect of SLS on the level of TGF-β1
Compared with the high glucose group: * P<0.05, ** P<0.01. Control group: DMEM with 5.5mmol/L glucose, high glucose group: DMEM with 30mmol/L glucose, S1 group: 5mg/ml SLS+30mmol/L glucose, S2 group: 10mg/ml SLS+30mmol/L glucose, S3 group: 20mg /ml SLS+30mmol/L glucose. SLS, Seed of Litchi chinensis Sonn.
Figure 2 Effect of SLS on the level of FN
Compared with the high glucose: * P < 0.05, ** P < 0.01. Control group: DMEM with 5.5mmol/L glucose, high glucose group: DMEM with 30mmol/L glucose, S1 group: 5mg/ml SLS+30mmol/L glucose, S2 group: 10mg/ml SLS+30mmol/L glucose, S3 group: 20mg/ml SLS+30mmol/L glucose. SLS, Seed of Litchi chinensis Sonn; FN, fibronectin.
Figure 3 Apoptosis detection by flow Annexin V-FITC/PI double staining
Control group: DMEM with 5.5mmol/L glucose, high glucose group: DMEM with 30mmol/L glucose, S1 group: 5mg/ml SLS+30mmol/L glucose, S2 group: 10mg/ml SLS+30mmol/L glucose, S3 group: 20mg /ml SLS+30mmol/L glucose. SLS, Seed of Litchi chinensis Sonn.
Figure 3 Effect of SLS on the expression of SOCS-1
a. AngII group: 10-6 mol/L Angiotension Ⅱ, b. AngII+SLS group: 20mg/L SLS and 10-6 mol/L AngⅡ, c. Control group: DMEM/F12 medium, d. High glucose+SLS group: 20mg/L SLS+25mmol/L of glucose, e. Control group: DMEM/F12 medium, f. High glucose group: 25mmol/L glucose. SOCS-1, suppressor of cytokine signaling-1. SLS, Seed of Litchi chinensis Sonn.
1.   Zhang AH, Huang SM, Ding GX, et al. JNK-c-Jun/AP-1 Signal Pathway Regulated AngiotensinⅡ-induced Human Mesangial Cell Proliferation. Acta Nanjing Med Univ 2004, 24(1): 4-8.
doi: 10.1007/s11670-004-0048-0
2.   Nishimoto N, Kishimoto T, Yoshizakik. Anti-interleukin6 receptor antibody treatment in rheumatic disease. Ann Rheum Dis 2000, 59(supple1): 121.
doi: 10.1136/ard.59.suppl_1.i21 pmid: 11053081
3.   Lou ZM, Tian JX, Wang WX, et al. Effect of total saponin extract from litchi core on the blood glucose levels of diabetic mice. Zhejiang Med J 2007, 29(6): 548-550.
4.   Kolset SO, Reinholt FP, Jenssen T.Diabetic nephropathy and extracellular matrix. J Histochem Cytochem 2012, 60(12): 976-986.
doi: 10.1369/0022155412465073 pmid: 23103723
5.   Li JW. Chinese Medical Dictionary (Second Edition). Beijing: People’s Med Publishing House 2013, 1184.
6.   Zhang YJ, Zhang C.Progress of litchi seeds on the main active ingredients and pharmacological effects. J Guangdong Pharm Univ 2014, 30(6):792-797.
7.   Jiang ZG, Ren K, Lin Z, et al. Study on the effective part of Litchi chinensis reducing blood glucose. J Changchun Univ Tradit Chin Med 2011, 27(1):14-16.
[1] Ji Yue, Yan Xue-Rou, Yang Hong-Tao, Yang Kang, Zhao Qing-Yun, Hu Shou-Ci, Su Qi-Hang. Influence of astragalus polysaccharide on kidney status and fibrosis indices of a rat model of streptozotocin-induced diabetic nephropathy[J]. Traditional Medicine Research, 2018, 3(4): 173-180.
[2] Tang Rui, Li Qia-Qia, Wang Di, Chen Jing, Huang Jin-Hua, Zeng Qing-Hai. The protective effect of Dendrobium officinale polysaccharides on photoaging fibroblasts by scavenging reactive oxygen species and promoting the expression of TGF-β1[J]. Traditional Medicine Research, 2018, 3(3): 131-139.
[3] Tao Zheng-Bo, Xiong Li-Yan, Wang Li-Hui, Zhang Chuan. Polysaccharide extracts of Cirsium japonicum protect rat H9c2 myocardial cells from oxidative stress induced by hydrogen peroxide[J]. Traditional Medicine Research, 2018, 3(3): 140-147.
[4] Yang Ming, Jin Yi, Yang Li-Ping. A systematic summary of natural compounds in Radix Glycyrrhizae[J]. Traditional Medicine Research, 2018, 3(2): 82-94.
[5] Xia Le-Min, Cui Le-Le, Jiang Yi-Ling, Zheng Qin, Zhang Ai-Ping, Luo Mei-Hong. Research on Xijiao Dihuang Decoction suppressing platelet apoptosis in immune-mediated aplastic anemia based on mitochondrial mediated pathway[J]. Traditional Medicine Research, 2017, 2(1): 27-32.