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1Traditional Medicine Research  2018, Vol. 3 Issue (3): 157-165    DOI: 10.12032/TMR201811074
Ancient Formula Research     
Effect of alternate-day-fasting combined with Lingguizhugan Decoction on blood lipid profiles of hyperlipidemic rats
Jun-Jie Zhang1,*(), Xian-Zhi He1, Guo-Shun Peng1, Zhen-Kun Wang1, Bin Ke2, Jian Qin2
1Department of Internal Medicine of Cardiology, Zhuhai Hospital of Traditional Chinese and Western Medicine, Zhuhai, China.
2Department of Traditional Chinese Medicine, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
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Highlights

Lingguizhugan Decoction didn’t enhance the effect of alternate-day-fasting in the prevention of hyperlipidemia.

Editor’s Summary

Effects of Chinese herb combined with behavior therapy in the treatment of hyperlipidemia need further exploration.

Abstract

Objective: To explore the effects of alternate-day-fasting (ADF) therapy combined with Lingguizhugan Decoction (LD) on blood lipid profiles of hyperlipidemic rats. Methods: Rats were randomly assigned into high-fat-diet (HF) group and normal-diet (ND) group. Hyperlipidemic rats fed with high-fat-diet for 5 weeks were randomly divided into ADF group, alternate-day-fasting with LD (ALG) group and model control (MC) group. The rats in ALG and ADF group were deprived of food for 24 h every other day for 4 weeks. Rats in ALG group were administrated with LD at fasting day. After 4 weeks of ADF therapy, plasma TC, TG, LDL-c and HDL-c were measured in each group. Expression of miR-143 and PPAR-γ protein from adipose was also analyzed. Results: When compared with MC group, after 4 weeks of ADF or combined ADF and LD therapy, the body weight was evidently reduced in ADF and ALG groups (P = 0.028, P = 0.036 by wk 8). The levels of plasma TC and TG decreased in ADF group and ALG group, which were significantly lower than those in MC group (P < 0.001, P = 0.045; P < 0.001, P = 0.005). However, the body weight and level of TC and TG in ALG group showed non-statistical difference in comparison with ADF group (ALG vs. ADF, P > 0.05). Expression of miR-143 and PPAR-γ were higher in MC group than that in NC group (P < 0.001). Compared with MC group, expression of miR-143 and PPAR-γ were significantly decreased in ADF (P = 0.038, P = 0.015) and ALG (P = 0.007, P < 0.001) groups. When compared with ADF group, expression of miR-143 and PPAR-γ were significantly decreased in ALG (P = 0.041, P = 0.046) group. Conclusion: ADF therapy alone not only reduced blood lipids, but also inhibited miR-143 and PPAR-γ protein expression in visceral adipose tissue. However, LD couldn’t reduce the levels of blood lipid profiles more effectively than using ADF alone. Perhaps the effects of LD combined with ADF in the prevention of hyperlipidemia need further exploration.



Key wordsAlternate-day-fasting      Lingguizhugan Decoction      Hyperlipidemia      MiR-143      PPAR-γ     
Published: 04 May 2018
Fund:  Funding: This study was supported by the grants from Guangdong International Cooperation Program (2012B050600019).
Corresponding Authors: Zhang Jun-Jie     E-mail: worrior1986@163.com.
About author: #These authors contributed equally to this work.
Cite this article:

Jun-Jie Zhang, Xian-Zhi He, Guo-Shun Peng, Zhen-Kun Wang, Bin Ke, Jian Qin. Effect of alternate-day-fasting combined with Lingguizhugan Decoction on blood lipid profiles of hyperlipidemic rats. 1Traditional Medicine Research, 2018, 3(3): 157-165. doi: 10.12032/TMR201811074

URL:

https://www.tmrjournals.com/tmr/EN/10.12032/TMR201811074     OR     https://www.tmrjournals.com/tmr/EN/Y2018/V3/I3/157

Figure 1 Flowchart
Animals randomization, modeling, intervention and analysis. Rats were randomized into high-fat-diet (HF) group and normal-diet (ND) group in the modeling phase. During period of intervention, rats in HF group were divided into model control (MC) group, alternate-day-fasting (ADF) group and alternate-day-fasting with Lingguizhugan Decoction (ALG) group. NC served as negative control group. Statistical analysis was performed on data from all animals.
Groups N Food intake(g/d) Calorie intake (kJ/d) Relative total-calorie intake
NC 10 15.03 ± 3.04 225.22 ± 52.84 100.0%
MC 8 19.95 ± 5.39a 347.06 ± 89.00b 141.6%
ADF 8 27.43 ± 2.69c 477.20 ± 44.77d 97.3%
ALG 8 27.81 ± 2.98e 483.87 ± 49.56f 98.6%
Table 1 Food intake, calorie and relative total-calorie intake
Groups 1-Wk 2-Wk 4-Wk 6-Wk
ND 299.3 ± 11.1 304.1 ± 14.4 346.2 ± 20.2 384.3 ± 18.0
HF 299.0 ± 16.3 340.1 ± 19.5a 412.7 ± 25.4b 442.3 ± 28.6b
Table 2 Effect of HF on body weight changes in rats (g, \(\overline{X}\) ± s)
Groups N 6-Wk 8-Wk 10-Wk
NC 10 384.3 ± 18.0 410.2 ± 22.5 426.6 ± 23.2
MC 8 444.3 ± 19.1a 459.4 ± 20.9b 477.6 ± 9.4c
ADF 8 437.1 ± 26.0d 414.4 ± 27.7e 392.3 ± 18.8fg
ALG 8 439.2 ± 20.5h 422.2 ± 11.8i 404.8 ± 13.7jk
Table 3 Effect of ADF or combined ADF and LD therapy on body weight changes (g, \(\overline{X}\) ± s)
Groups TC TG HDL-c LDL-c
ND 1.29 ± 0.47 0.51 ± 0.22 0.46 ± 0.18 0.12 ± 0.06
HF 2.17 ± 0.25a 0.94 ± 0.29a 0.86 ± 0.09a 0.47 ± 0.08a
Table 4 Effect of HF on blood lipid profiles (mmol/L, \(\overline{X}\) ± s)
Groups TC TG HDL-c LDL-c
NC 1.70 ± 0.27 0.65 ± 0.29 0.53 ± 0.10 0.23 ± 0.09
MC 3.56 ± 0.75a 1.29 ± 0.19b 0.77 ± 0.37 0.53 ± 0.21c
ADF 2.21 ± 0.33d 1.16 ± 0.42e 1.01 ± 0.12f 0.51 ± 0.13g
ALG 2.01 ± 0.31h 0.81 ± 0.25i 0.94 ± 0.10j 0.51 ± 0.09k
Table 5 Effect of ADF or combined ADF and LD therapy on blood lipid profiles (mmoL/L, \(\overline{X}\) ± s)
Figure 2 Relative miR-143 expression in adiposity tissue
a, MC vs. NC <i>P </i>< 0.001; b, ADF vs. MC <i>P </i>= 0.038; c, ALG vs. MC <i>P </i>= 0.007; d, ALG vs. ADF <i>P </i>= 0.041.
(NC: negative control, MC: model control, ADF: alternate-day-fasting, ALG: alternate-day-fasting group with LD)
Figure 3 PPAR-γ/GADPH expression in adiposity tissue
a, MC vs. NC, <i>P </i>< 0.001; b, ADF vs. MC <i>P </i>= 0.015; c, ALG vs. MC <i>P </i>< 0.001; d, ALG vs. ADF <i>P </i>= 0.046.
(NC: negative control, MC: model control, ADF: alternate-day-fasting, ALG: alternate-day-fasting group with LD)
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