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Traditional Medicine Research  2017, Vol. 2 Issue (3): 149-154    DOI: 10.12032/TMR201707052
Special Therapy of Traditional Medicine     
Fireneedle up-regulates the expression of ERK1/2 in neural stem cells
Xu Jia-Chun1, Zhao Zhi-Heng1, Cheng Su-Li1, Wang Xue1, Jiao Zhao-Hua2, Cai Zhi-Min1,3, Zhou Zhen4, Liu Bao-Hong2, Li Yan2,*()
1Tianjin University of Traditional Medicine Research, Tianjin, China.
2Tianjin Gongan Hospital, Tianjin, China.
3Hebei Normal University of Science & Technology, Hebei, China.
4The 2nd Affiliated Hospital of Tianjin University of TCM, Tianjin, China.
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Highlights

Fire needlerepairs the spinal cord injury via promoting neural stem cells differentiate into neurons.

Editor’s Summary

The earliest record of ancient literature about fire needle was in Lingshu, which was published in warring state period of China (475 B.C. - 221 B.C.).

Abstract

Objective: To explore the effect of fire needle on the expression of ERK1/2 of neural stem cells (NSCs) obtained from rats with spinal cord injury (SCI). Methods: Sixty SD female rats were randomly divided into 5 groups, including 5 in the blank group, 5 in the sham operation group, 25 in the model group, and 25 in the fire needle treated group. Rats in the model group and the fire needle treated group were further divided into the 1d group, the 3d group, the 7d group, the 10d group and the 14d group, respectively. Modified Allen’s method was applied to build SCI model, and serum in the abdominal aorta was collected at the indicated time and placed into NSCs culture dishes for 72h. RT-PCR was applied to detect the expression of ERK1/2. Results: According to the Basso, Beattie & Bresnahan scale (BBB scale), rats in the model group and the fire needle treated group scored 0 at first day, suggesting a success of modeling after treatment. The scores of the fire needle group were significantly higher than those in the model group (P < 0.05). Compared with sham operation group, the expression of ERK1 and ERK 2 in model group and fire needle group were significantly decreased at day 1 (P < 0.05), and lasted for 3d (P < 0.05). At day 7 and day 10, the expressions of ERK1 and ERK2 in the fire needle group were significantly higher than those in the model group (both P < 0.05). Conclusion: Fire needle could up-regulate the expression of ERK1 and ERK2of NSCsfrom SCI rats.



Key wordsFire needle      Spinal cord injury      ERK      Neural stem cells     
Published: 05 July 2017
Fund:  This work was supported by National Natural Science Foundation (NO.81373839).
Corresponding Authors: Li Yan     E-mail: liyan66950@sina.com
About author: Executive Editor: Cui-Hong Zhu English Editor: Lin Li, Li-Yun-Cui, Ya-Song Wang, Xiao-Hua Zhang, Xiao-Dong Wang, Hong-Xu Zhu, Zhen-Yan Liu, Yi-Cheng Shi
Cite this article:

Xu Jia-Chun, Zhao Zhi-Heng, Cheng Su-Li, Wang Xue, Jiao Zhao-Hua, Cai Zhi-Min, Zhou Zhen, Liu Bao-Hong, Li Yan. Fireneedle up-regulates the expression of ERK1/2 in neural stem cells. Traditional Medicine Research, 2017, 2(3): 149-154. doi: 10.12032/TMR201707052

URL:

https://www.tmrjournals.com/tmr/EN/10.12032/TMR201707052     OR     https://www.tmrjournals.com/tmr/EN/Y2017/V2/I3/149

Figure 1 NSCs culture
The purified cells were identified by Nestin immunoreaction. NSCs, Neural stem cells.
Figure 2 BBB scores of rats
# indicates the difference is statistically significant compared with sham operation group (P < 0.05). * indicates the difference at the same time point is statistically significant between model group and fire needle group (P < 0.05). BBB scale, Basso, Beattie & Bresnahan scale.
Figure 3 ERK1 gene expression
#indicates the difference is statistically significant compared with sham operation group (P <0.05). *indicates the difference at the same time point is statistically (P <0.05) between fire needle group and model group. @indicates the difference is statistically significant compared with that at former time point in the same group (P <0.05). ERK, Extracellular signal-regulated kinase.
Figure 4 ERK2 gene expression
#indicates the difference is statistically significant compared with sham operation group (P <0.05). * indicates the difference at the same time point is statistically significant between model group and fire needle group (P <0.05). @indicates the difference is statistically significant compared with that at former time point in the same group (P <0.05). ERK, Extracellular signal-regulated kinase.
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