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05 July 2016, Volume 1 Issue 3 Previous Issue    Next Issue
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Orginal Article
Traditional Chinese medicine should not be ignored during the development of precision medicine with Chinese characteristics
Shang Hong-Cai, Wu Xiong-Zhi, Qiu Rui-Jin, Wang Ya-Han, Wang Yong-Yan
Traditional Medicine Research. 2016, 1 (3): 105-114.   https://doi.org/10.12032/TMR201603015
Abstract ( 360 )   HTML ( 6 )     PDF (323KB) ( 263 )  

Highlights:

TCM has been playing an important role in disease treatment for thousands of years. This study presents that it may contribute to the development of precision medicine in China, if the prevention, diagnosis and treatment in TCM develop to precision.

Precision medicine will become a hot issue in future’s medical researches. Traditional Chinese medicine (TCM) has been playing an important role in disease treatment for thousands of years. Developing precision medicine also needs TCM. Preventing disease should adjust according to time, circumstances and the variability of an individual treatment with TCM. The TCM disease name, syndrome differentiation type, the name of the syndrome, and syndrome diagnostic criteria should be standardized and unified. It needs further studies about precision corresponding of prescription and medicine, the correspondence of prescription and syndrome, the relation of dose-effect/toxic-effect in TCM, new drug design and creation, improving the quality standard of TCM, so that TCM may achieve the aim of precision prevention, precision diagnosis and precision treatment.

The present treatment and mechanism progress of arsenic trioxide in hematological malignancies
Liu Lin, Zhao Zhi-Gang
Traditional Medicine Research. 2016, 1 (3): 115-121.   https://doi.org/10.12032/TMR201603016
Abstract ( 318 )   HTML ( 2 )     PDF (215KB) ( 257 )  

Highlights:

This paper reviewed the development history of arsenic trioxide (ATO), and summarized its mechanisms and clinical application in the treatment of hematologic malignancies. ATO can induce tumor cell apoptosis, tumor cell differentiation and play a role in the treatment of malignant tumor of the blood system.

Arsenic trioide (ATO) is a kind of highly toxic substances and the main effective components of arsenic. ATO has been used medicinally for over 2400 years. Recent years, many studies have found that ATO has a significant efficacy on the acute promyelocytic leukemia (APL). To further explore the latest findings of research on the treatment of hematologic malignanies of ATO, this paper reviewed the development history of ATO, and summarized its mechanisms and clinical application in the treatment of hematologic malignancies. In addition, we want to provide new ideas for researches on the treatment of cancer of ATO.

Molecular docking study on the molecular mechanism of rhaponticin for treatment of chronic myelocytic leukemia
Liu Li-Juan, Zhou Chao, Song Fu-Cheng, Zhang Sen-Sen, Teng Wen-Jing, Zhuang Jing, Sun Chang-Gang, Si Hong-Zong
Traditional Medicine Research. 2016, 1 (3): 122-127.   https://doi.org/10.12032/TMR201603017
Abstract ( 391 )   HTML ( 4 )     PDF (317KB) ( 378 )  

Highlights:

Molecular docking can not only design drugs for the targets, but also detect mechanism of drug effecting on the targets. This study researched the interaction of rhaponticin and genes associated with chronic myelocytic leukemia (CML). The results showed JUN (2G01) was the preference receptor for rhaponticin and SRC (SRC), JAK2 (5AEP), MAPK14 (2YIX), FRAP1 (3OAW), MAPK8 (3PZE), PARP1 (1U5Y) had high scores.

To study the mechanism of rhaponticin treating chronic myelocytic leukemiathe using molecular docking to analyze the interaction of rhaponticin and genes. We screened the genes associated with chronic myelocytic leukemia as the receptors on the basis of our previous network pharmacology study, and observed the results of molecular docking respectively with rhaponticin by software Surflex-Dock. We got results of molecular docking score and hydrogen bond between rhaponticin and 19 genes associated with chronic myelocytic leukemia. We could infer that JUN (2G01) was the preference receptor for rhaponticin and SRC (SRC), JAK2 (5AEP), MAPK14 (2YIX), FRAP1 (3OAW), MAPK8(3PZE), PARP1 (1U5Y) had high scores. The treatment of rhaponticin for chronic myelocytic leukemia is multiple targets and multiple ways.

Computational study of the molecular mechanism of Lonicera japonica organic acids against influenza
Luo Xuan, Shen Xia, Hu Ben-Xiang, Wang Hao, Zhao Zhen-Yu, Guo Zi-Hu
1Traditional Medicine Research. 2016, 1 (3): 128-137.   https://doi.org/10.12032/TMR201603018
Abstract ( 395 )   HTML ( 3 )     PDF (622KB) ( 572 )  

* Xia Shen and Xuan Luo contributing equally to this work.

Highlights:

1. Molecular docking was applied for determining the potential targets of Lonicera japonica organic acids.
2. Finding relationships between the organic acids and proteins of influenza virus NA, HA, M2 and PB2.
3. Lonicera japonica against influenza virus by multiple components with synergistic effect.
4. Provided molecular evidences on treatment of influenza virus infection with Lonicera japonica and a modern theory for anti-influenza virus treatment by natural herbs.

Objective: High evolutionary rate by mutation or by re-assortment makes influenza virus a threat to human health. Recently, many studies have shown that herbal medicine Lonicera japonica is effective against influenza virus infection. However, owing to the natural herb’s complexity in both components and functions, its molecular mechanism is not fully understood. Here, we use computational methods to study the molecular mechanism of anti-influenza activity of organic acids in Lonicera japonica. Methods: Molecular docking was applied for determining the potential targets of the organic acids. Moreover, the compound-target networks were constructed to clarify the relationship between organic acids and their relative targets. Results: Each compound had inhibitory activity against all tested targets. In addition, three molecules (D, E and F) from Lonicera japonica were predicted as potential inhibitors of the mutants of N1 and N9 subtypes of influenza viruses. Conclusions: Our study provided molecular evidences on treatment of influenza virus infection with Lonicera japonica and a modern theory for anti-influenza virus treatment by natural herbs. We confirmed that Lonicera japonica acts synergistically with multiple components against influenza virus. Three molecules (D, E, and F) were selected and predicted as potential inhibitors of N1 and N9 as well as their mutants.

Orginal Article
Uncertainty profile for NIR analysis of tanshinone I content in tanshinone extract powders
Xue Zhong, Xu Bing, Yang Chan, Wang Xin, Sun Fei, Shi Xin-Yuan, Qiao Yan-Jiang
Traditional Medicine Research. 2016, 1 (3): 138-148.   https://doi.org/10.12032/TMR201603019
Abstract ( 361 )   HTML ( 3 )     PDF (502KB) ( 239 )  

Highlights:

A rapid, non-destructive and reliable NIR method was developed and validated for the quantification of tanshinone I content in Salvia miltiorrhiza extract. After spectral pretreatment, different variable selection methods were used to select sensitive variables and develop partial least squares regression models. A novel approach based on uncertainty profile for NIR method was applied to control the quality of tanshinone extract and provide reference for quality control of other Chinese herbal medicine.

A rapid, non-destructive and reliable analytical method using NIR diffuse reflectance spectroscopy combined with variable selection methods was developed and validated for the quantification of tanshinone I content in tanshinone extract. After spectral pretreatment, different variables selection methods such as interval partial least square (iPLS), synergy interval partial least square (SiPLS), uninformative variables elimination (UVE), successive projections algorithm (SPA) and competitive adaptive reweighted sampling (CARS) were used to select sensitive variables and to develop partial least squares (PLS) regression models. Results indicated that UVE method was an efficient method to eliminate the redundant information, extract useful features and improve model performance. The root mean squared error of prediction (RMSEP) and ratio of performance to deviation (RPD) of the quantitative model were improved form 0.483% and 12.26 to 0.433% and 13.67, respectively. A global strategy was proposed to examine the validity of the built NIR method as well as to estimate its uncertainty at the same time. And an innovative formula we offered to assess the uncertainty was based on the calculation of the β-content tolerance interval by the Hoffman-Kringle approach. Furthermore, a novel approach based on uncertainty profile (UP) was used to validate the robustness and accuracy of PLS model. It concluded that NIR analysis combined with variables selection method was suitable and reliable for quantification of tanshinone I content in tanshinone extract, and could be applied to control the quality of tanshinone extract and provide reference for quality control of other Chinese herbal medicines.

Feiji Recipe Reverses Lung Cancer Immune Escape by Inhibiting the Expression of IDO
Que Zu-Jun, Bi Ling, Zhou Zhi-Yi, Zhou Lei, Wang Qing, Luo Bin, Liu Jian-Wen, Li He-Gen, Tian Jian-Hui
Traditional Medicine Research. 2016, 1 (3): 149-155.   https://doi.org/10.12032/TMR201603020
Abstract ( 463 )   HTML ( 7 )     PDF (356KB) ( 223 )  

Tumor cells have developed multiple mechanisms to escape immune recognition mediated by T cells. Indoleamine 2,3-dioxygenase (IDO), a tryptophan-catabolizing enzyme inducing immune tolerance, is involved in tumor escape from host immune systems. Feiji Recipe, a Chinese herbal recipe that composed of 12 traditional Chinese medicines, has been used to recover immuno-surveillance and to interven immune escape in lung cancer patients. To elucidate the biological role of IDO in the tumorigenesis of lung cancer, and the intervention of Feiji Recipe on tumor immune escape, we established a mouse lung cancer cell line (2LL-EGFP-IDO) with overexpression of IDO. IDO expression did not affect cell proliferation. 2LL-EGFP-IDO cells were effective in reducing the level of tryptophan and increasing the level of its downstream metabolites, kynurenines, in culture medium as assessed by HPLC. Feiji Recipe could up-regulate the percentage of CD8+ T cells by inhibits the expression of IDO in co-culture system. The results of these studies indicate that Feiji Recipe can enhance the immune response and reverses tumor immune escape by induce the activity of CD8+ T cells, which might be related to the inhibition of IDO expression.

Orginal Article
Explore the medication rules of Chinese medicine of Professor Huang Wenzheng for the treatment of chronic kidney disease based on data mining
Dong Shao-Ning, Wang Yao-Guang, Xu Bing, Jin Liang, Wang Cui-Han
Traditional Medicine Research. 2016, 1 (3): 156-165.   https://doi.org/10.12032/TMR201603021
Abstract ( 472 )   HTML ( 3 )     PDF (619KB) ( 255 )  

Highlights:

The study found the backbone network and three-level network of the Chinese medicine for the treatment of CKD by homogeneous network analysis. The 5 top commonly used herbs were Dan Shen (Radix Salviae Miltiorrhizae) , Fu Ling (Poria) , Huang Qin (Radix Scutellariae), Sheng Di Huang (Radix Rehmanniae Recens) and Sha Ren (Fructus Amomi Villosi), respectively.

In recent years, chronic kidney disease (CKD) received increased attention due to its high prevalence and adverse effects. Current treatment options for CKD are limited. Many patients seek alternative therapies, such as traditional Chinese medicine (TCM). TCM has been used widely in the treatment CKD for many years. The purpose of this study is to explore the medication rules of Professor Huang Wenzheng's treatment of CKD with Chinese medicine based on data mining. The structured case database was built for 131 patients with CKD treated by Prof. Huang. The complex network analysis was applied to analyze the medication principle of Chinese medicines.The study found the backbone network and three-level network of the Chinese medicines for the treatment of CKD by homogeneous network analysis. The 5 top commonly used herbs were Dan Shen (Radix Salviae Miltiorrhizae) , Fu Ling (Poria) , Huang Qin (Radix Scutellariae), Sheng Di Huang (Radix Rehmanniae Recens) and Sha Ren (Fructus Amomi Villosi), respectively.

Ban Xia Lian Qiao Tang
Yan Da-Zhi
Traditional Medicine Research. 2016, 1 (3): 166-167.   https://doi.org/10.12032/TMR201603022
Abstract ( 263 )   HTML ( 2 )     PDF (197KB) ( 257 )  

Composition

Jiang Ban Xia (Rhizome Pinelliae Fermentata) 30g, Lian Qiao (Fructus Forsythiae) 30g

Directions The herbs are decocted with 500ml water and concentrated to 200ml. The patients between the ages of five and twelve take one-third-dose, while twelve years old and over take the whole dose. Ban Xia Lian Qiao Tang (BXLQT) is taken twice daily, half an hour after meals while the decoction is warm. BXLQT also can be made as granules, which are taken with the same method. Mild cases take three days, while serious cases take seven days to recover sore throat and throat discomfort.

Indications This prescription is mainly applied to treat all kinds of sore throat and throat discomfort, which are named sore throat, globus hysteriocus and sore throat dumb in Traditional Chines Medicine, and pharyngitis in Western Medicine. Herbs can be added according to the differentiation of diseases and syndrome for the external and internal diseases.

Contraindications

The patients who are less than 5 years old are not recommended to take the BXLQT. Women who want to conceive or are pregnant, especially during the first trimester of pregnancy, should not take the BXLQT.

2019-nCoV

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